Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His Tag&Tag Free

• Background:

  Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

• Description:

  Human ITGAV&ITGB5 Heterodimer Protein, His Tag&Tag Free (IT5-H52W5) is expressed from human 293 cells (HEK293) . It contains AA Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5) (Accession # NP_002201.1 (ITGAV) & NP_002204.2 (ITGB5) ) .

• Specifications:

  Human ITGAV&ITGB5 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 112.9 kDa (ITGAV) and 81.9 kDa (ITGB5) . Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 85-95 kDa (ITGB5) respectively due to glycosylation.

• Accession Number:

  NP_002201.1 (ITGAV) & NP_002204.2 (ITGB5)

• Expression Region:

  Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5)

• Host:

  HEK293

• Target:

  Integrin alpha V beta 5

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis | Native

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Vitronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB5 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT5-H52W5) with a linear range of 2-20 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Tris, 150 mM NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  112.9 kDa (ITGAV) & 81.9 kDa (ITGB5)

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  100ug*1

• Species:

  Human