Exo1, exoglucanase isoenzyme 1

• Catalog number
  AS08 322
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Lyophilized
• Immunogen
  synthetic peptide conjugated to KLH
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 100 µl of sterile water
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  Western blot (WB), ELISA (ELISA)
• Connected products
  no related to other products
• Recommended dilutions for use
  1: 5 000 with standard ECL (WB) , 1: 10 000 (ELISA)
• Molecular weight expected аpparent
  66 kDa
• Verified reactivity
  Hordeum vulgare
• Possible reactivity
  Oryza sativa, Zea mays
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  to be added when available
• References
  to be added when available
• Scientific context
  Family 3 beta-d-glucan glucohydrolases are widely distributed in higher plants. The enzymes catalyse the hydrolytic removal of beta-d-glucosyl residues from non-reducing termini of a range of beta-d-glucans and beta-d-oligoglucosides. Their broad specificity can be rationalized from X-ray crystallographic data obtained from a barley beta-d-glucan glucohydrolase in complex with non-hydrolysable S-glycoside substrate analogues, and from molecular modelling of enzyme-substrate complexes. The glucosyl residue occupying binding subsite -1 is tightly locked into a fixed position through extensive hydrogen bonding with six amino acid residues near the bottom of an active site pocket. In contrast, the glucosyl residue at subsite +1 is located between two tryptophan residues at the entrance of the pocket, where it is less tightly constrained. The relative flexibility of binding at subsite +1, coupled with the projection of the remainder of bound substrate away from the enzyme-™s surface, means that the overall active site can accommodate a range of substrates with variable spatial dispositions of adjacent beta-d-glucosyl residues. The broad specificity for glycosidic linkage type would enable the enzyme to perform diverse functions during plant development.
• Notes
  None
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink

• Gene target
  Exo1   exoglucanase   isoenzyme  
• Short name
  Exo1, exoglucanase isoenzyme 1
• Alternative name
  exonuclease 1, exoglucanase isoenzyme 1
• Alternative to gene target
  exonuclease 1, HEX1 and hExoI, EXO1 and IDBG-107718 and ENSG00000174371 and 9156, double-stranded DNA specific 5'-3' exodeoxyribonuclease activity, nuclei, Exo1 and IDBG-206570 and ENSMUSG00000039748 and 26909, EXO1 and IDBG-631406 and ENSBTAG00000009396 and 506390

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