Human Superoxide Dismutase Recombinant

• Background: Cu/Zn Superoxide Dismutase (SOD1) antagonizes IL3 dependent proliferation of cells in culture and reversibly inhibits DNA synthesis of erythroid progenitor cells. SOD1 binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. SOD1 is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.
• Description: SOD is a disulfide-linked homodimeric protein consisting of two 154 amino acid residues, and migrates as an approximately 31 kDa protein under non-reducing and as 16 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Superoxide Dismutase mature chain was expressed inE. coli.
• UniProt: P00441
• Endotoxin: < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
• Purity: ≥ 95% by SDS-PAGE and HPLC
• Format: Lyophilized protein
• Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
• Shipping Conditions: Gel Packs or Dry Ice
• Storage Conditions: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
• Calculated Molecular Weight: 16 kDa
• Formulation: Lyophilized from a 0.2 µm filtered PBS solution, pH 7.0.