Human BCA1 (CXCL13) Recombinant

• Background: The homeostatic chemokine CXCL13 (also called B cell- attracting chemokine 1 [BCA- 1] or B-lymphocyte chemoattractant [BLC]) is constitutively expressed in secondary lymphoid tissue and initiates lymphoid neogenesis when expressed aberrantly in mice. CXCL13 has also been detected in chronic inflammation associated with human lymphoid neogenesis, suggesting a pathogenic role. Follicular dendritic cells (FDCs) are generally considered to be the major source of CXCL13 both in normal and aberrant lymphoid tissue. CXCL13 has been the exclusive ligand for CXCR5, which initiates chemotaxis toward an increasing gradientin vitroand to B cell zones in lymph nodes that express CXCL13. More recently, CXCR5 expression has also been shown in DN T cells in mice, a subset of CD4 follicular Th cells, and some tonsillar CD4+CD25+ Treg cells in humans.
• Description: Recombinant human BCA1 (B cell- attracting chemokine 1), also known as CXCL13 or BLC, is a disulfide-linked monomeric protein consisting of 88 a.a. and migrates as an approximately 10 kDa protein under reducing and non-reducing conditions. Human CXCL13, generated by the proteolytic removal of the signal peptide and propeptide, has a calculated molecular mass of ~10 kDa. Optimized DNA sequence encoding human BCA1 mature domain was expressed inE. coli.
• UniProt: Q53X90
• Endotoxin: Endotoxin level was found to be < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
• Purity: ≥ 98% by SDS-PAGE and HPLC
• Format: Lyophilized protein
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/ml. This solution can then be diluted into other buffers.
• Shipping Conditions: Gel Packs or Dry Ice
• Storage Conditions: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at 2 - 8° C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
• Calculated Molecular Weight: 10 kDa
• Formulation: Lyophilized from a 0.2 µm filtered solution containing 20 mM phosphate buffer, pH 7.5,100 mM NaCl.