DNA Polymerase β (POLB), His-tag Recombinant

• Background:

  DNA polymerase involved in base excision and repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The protein is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage.

• Description:

  Human DNA polymerase β (beta), also known as Pol beta and POLB, GenBank Accession No. NM_002690, a.a. 2-335 (end), with N-terminal His-tag, expressed in anE. colicell expression system. MW=39 kDa.

• UniProt:

  P06746

• Tag:

  N-terminal His-tag

• Purity:

  ≥ 80%

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at -80°C.

• Calculated Molecular Weight:

  39 kDa

• Formulation:

  40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 250 mM imidazole, 0.04% Tween-20, and 20% glycerol.