Mouse ACE(Angiotensin Ⅰ Converting Enzyme) EIA Kit
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Catalog number
E-EL-M0091-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Mouse
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Target Synonym
ACE1, CD143, DCP, DCP1, ICH, MVCD3
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ACE. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ACE. You can calculate the concentration of Mouse ACE in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.19 ng/mL
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Detection Range
0.313~20ng/mL
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Target Name
ACE
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UNIProt ID
P09470
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Mouse ACE(Angiotensin Ⅰ Converting Enzyme) EIA Kit
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Type
Sandwich
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Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C. Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Gene target
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Gene symbol
ACE
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Short name
Mouse ACE(Angiotensin Ⅰ Converting Enzyme) EIA Kit
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Technique
Enzyme, Mouse, EIA, eia, enzymes, mouses
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Host
mouse
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Species
Mouses
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Alternative name
Mouse Angiotensin-converting enzyme; Uncharacterized protein(Angiotensin Ⅰ Converting Enzyme) EIA reagent
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Alternative technique
enzymes, murine, kits, elisas
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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