PRO-PREP Protein Extraction Solution (Cell/Tissue) (EDTA free)

Type

Solution
Application

Protein Prep.
ENApiry date

2 years
Shippin Tem

D/B
Category

Protein
Description

For cells, cell lines and tissues in culture till half confluency. The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous. 6
Buffer

Plasmas are treated with K2, K3 or Na EDTA disodium chelator at pH8. The Ethylene-diamine-tetra acetic acid with Cas nr 60-00-4 is called Edetic acid. The chemical formula is C10H16N2O8. As anticoagulant GENTAUR also uses heparin and sodium citrate to produce plasmas.

Gene target

PRO-PREP Protein Extraction Tissue free
Gene symbol

PREP, PITRM1, TRP-AGG5-1, TRP-AGG2-1, TRP-AGG3-1, TRP-TGG1-1, TRP-AGG6-1, TRP-CGG1-3, TRP-TGG2-1, TRP-TGG4-1
Short name

PRO-PREP Protein Extraction ( /Tissue) ( free)
Technique

tissue, tissues
Alternative name

L-proline-preparation reagent Protein Extraction solution (cellular/Tissue) (EDTA free)
Alternative technique

tissues
Tissue

cell, tissue

Identity

HGNC:34578
Gene

TRP-AGG2-1
Long gene name

tRNA-Pro (anticodon AGG) 2-1
Synonyms gene
- TRNAP4
Synonyms gene name
- transfer RNA proline 4 (anticodon AGG)
- transfer RNA-Pro (AGG) 2-1
Synonyms
- tRNA-Pro-AGG-2-1
GenBank acession
- HG983978
Locus

1q24.2
Discovery year

2008-08-29
Entrez gene record

100189019
Classification
- Cytoplasmic transfer RNAs

Identity

HGNC:34929
Gene

TRP-AGG3-1
Long gene name

tRNA-Pro (AGG) 3-1
Synonyms gene
- TRNAP20
Synonyms gene name
- transfer RNA proline 20 (anticodon AGG)
- transfer RNA-Pro (AGG) 3-1
Synonyms
- tRNA-Pro-AGG-3-1
GenBank acession
- HG983986
Locus

16p13.3
Discovery year

2008-08-29
Entrez gene record

100189361
Classification
- Low confidence cytoplasmic transfer RNAs

Identity

HGNC:34852
Gene

TRP-TGG1-1
Long gene name

tRNA-Pro (anticodon TGG) 1-1
Synonyms gene
- TRNAP17
Synonyms gene name
- transfer RNA proline 17 (anticodon UGG)
- transfer RNA-Pro (TGG) 1-1
Synonyms
- tRNA-Pro-TGG-1-1
GenBank acession
- HG983995
Locus

14q11.2
Discovery year

2008-08-29
Entrez gene record

100189286
Classification
- Cytoplasmic transfer RNAs

Identity

HGNC:34686
Gene

TRP-CGG1-3
Long gene name

tRNA-Pro (anticodon CGG) 1-3
Synonyms gene
- TRNAP8
Synonyms gene name
- transfer RNA proline 8 (anticodon CGG)
- transfer RNA-Pro (CGG) 1-3
Synonyms
- tRNA-Pro-CGG-1-3
GenBank acession
- HG983992
Locus

17p13.1
Discovery year

2008-08-29
Entrez gene record

100189124
Classification
- Cytoplasmic transfer RNAs

Identity

HGNC:34787
Gene

TRP-TGG2-1
Long gene name

tRNA-Pro (anticodon TGG) 2-1
Synonyms gene
- TRNAP14
Synonyms gene name
- transfer RNA proline 14 (anticodon UGG)
- transfer RNA-Pro (TGG) 2-1
Synonyms
- tRNA-Pro-TGG-2-1
GenBank acession
- HG983996
Locus

11q13.5
Discovery year

2008-08-29
Entrez gene record

100189224
Classification
- Cytoplasmic transfer RNAs

Identity

HGNC:35056
Gene

TRP-TGG4-1
Long gene name

tRNA-Pro (anticodon TGG) 4-1
Synonyms gene
- TRNAP26P
Synonyms gene name
- transfer RNA proline 26 (anticodon UGG) pseudogene
- transfer RNA-Pro (TGG) 4-1
Synonyms
- tRNA-Pro-TGG-4-1
GenBank acession
- HG984002
Locus

16p13.3
Discovery year

2008-08-29
Entrez gene record

100189483
RefSeq identity
- NG_008541
Classification
- Cytoplasmic transfer RNA pseudogenes

Name

Tissue Fixation
Concept

Scope note: The technique of using FIXATIVES in the preparation of cytologic, histologic, or pathologic specimens for the purpose of maintaining the existing form and structure of all the constituent elements.
Tree numbers
- E01.370.225.500.620.760.720
- E01.370.225.750.600.760.720
- E05.200.500.620.760.720
- E05.200.750.600.760.720
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data