For quantitative measurement of mouse CD117 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
ELISA standard curve
Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.020 + 0.074 + 0.156 + 0.273 + 0.583 + 1.070 + 1.856 + 2.625
ELISA kit detection limits
156pg/ml to 10000pg/ml
ELISA's sensitivity< 10pg/ml
Target of this ELISA
Natural and recombinant mouse CD117
the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);860;1958;5559;978;2148;6579 ELISA standard deviations 41.28;107.69;339.10;43.03;135.32;578.95 CV(%);4.8;5.5;6.1;4.4;6.3;8.8
ELISA kit description
GENTAUR's mouse CD117 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for CD117 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S25-P527) and your samples are measured with a biotin conjugated polyclonal from goat specific for CD117 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse CD117 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies
A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse CD117 antibodies;1 ST0000-10;Lyophilized recombinant mouse CD117 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse CD117 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
heparin or EDTA
How the elisa works
Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse CD117 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, cell lysate, tissue homogenates, serum or plasma (heparin, EDTA) to the ELISA plate. Keep in mind that each mouse CD117 standard solution and each sample be measured in duplicate.
Minutes of TMB incubation
More gene info
SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Mast/stem cell growth factor receptor Kit
C Kit ; CD 117 ; CD117 ; ckit ; KIT oncogene ; Mast ; P10721 ; p145 c-kit ; PBT ; Piebald trait protein ; Proto oncogene c Kit ; Proto-oncogene c-Kit ; SCFR ; soluble KIT variant 1 ; Steel Factor Receptor ; Stem cell factor receptor ; Tyrosine-protein kinase Kit
Gen protein identity
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mouse CD117/c-kit PicoKine ELISA Kit
ELISA kit, ELISA, Mouse
Mouse CD117/c-reagent PicoKine Enzyme-linked immunosorbent assay reagent
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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