Multiplex ELISA Kit For Human Cytokine Panel 1 (6-Plex)

• Description:

  Product Overview

  The Multiplex Human Cytokine Panel 1 (6-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of six biomarkers or analytes (IL-1β, IL-6, TNFα, IFN-γ, IL-1α, IL-10).

  Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing six distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.

  Using only 25 µl of sample per well, up to 80 samples can be assayed for all six markers in the panel in 2.25 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

  Assay Ranges

  Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
  IFNγ	Sandwich	8000 -10.97 (pg/mL)	5600 (pg/mL)	14.3 (pg/mL)	4.95 (pg/mL)
  IL-1α	Sandwich	4000 -5.49 (pg/mL)	2800 (pg/mL)	7.1 (pg/mL)	5.43 (pg/mL)
  IL-1β	Sandwich	12226 -16.77 (pg/mL)	8558 (pg/mL)	21.8 (pg/mL)	12.40 (pg/mL)
  IL-6	Sandwich	3000 – 4.12 (pg/mL)	2100 (pg/mL)	5.3 (pg/mL)	3.47 (pg/mL)
  IL-10	Sandwich	8039 – 11.03 (pg/mL)	5627 (pg/mL)	14.3 (pg/mL)	10.99 (pg/mL)
  TNFα	Sandwich	6500 – 8.92 (pg/mL)	4522 (pg/mL)	11.5 (pg/mL)	7.74 (pg/mL)

  Kit Components

  Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.

  Reagents Include:

  Calibrator: Lyophilized, recombinant antigens in a buffered protein base
  Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
  Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
  Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
  Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
  Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
  Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
  Plate Seals (3): Adhesive strips

• CAS Number: 7732-18-5