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Category
Primary Antibodies
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Long description
Activation of NF-?B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2). Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR (3,4). Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase (5). TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation (5).
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Antibody come from
Hybridoma produced by the fusion of splenocytes from mice immunized with synthetic peptide corresponding to N terminal extracellular domain amino acids 1-29 of Human GnRHR and mouse myeloma cells.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti TRIAD3A (TLR-Ubiquitinating Enzyme) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Mouse
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Antibody can be used for Western blotting (1-2 µg/ml), immunofluorescence, flow cytometry, immunohistochemistry on formalin fixed, paraffin embedded tissues (1:100 dilution for 60 minutes after boiling in 1 mM EDTA for 10-20 min followed by colling to RT for 20 min). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Karande AA et al. Establishment of immunological probes to study human gonadotropin-releasing hormone receptors. Mol Cell Endocrinol 114:51-6 (1995)._x000B__x000B_2. Wolfahrt, S., et al. 'Endogenous regulation of the GnRH receptor by GnRH in the human placenta.' Mol Hum Reprod, 7, 89-95 (2001)._x000B__x000B_3. Rajeshwari, K., et al. 'Molecular mimicry by antiidiotypic monoclonal antibody to gonadotropin releasing hormone.' Immunol Invest, 28, 103-114 (1999)._x000B__x000B_4. Cheng, C.K., et al. 'Characterization of a new upstream GnRH receptor promoter in human ovarian granulosa-luteal cells.' Mol Endocrinol, 16, 1552-1564 (2002).
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
