Clathrin, Clathrin heavy-chain 1,2 (40 µg, HRP-conjugated)
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Catalog numberAS10 690-HRP
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Price:
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Size40 µg
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Available ordering formatLiquid, conjugated to HRP
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ImmunogenKLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationAffinity purified serum
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How to reconstituteSee included datasheet or contact our support service
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Storage conditionStore at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
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Verified applicationswestern blot (WB), immunolocalization (IL), immunofluorescence (IF)
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Connected productsAS10 680 | Tubulin alpha chainAS10 681 | Tubulin beta chainPlant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use1: 2000 with standard ECL (WB), 1: 2400 (IL), 1:400 (IF)
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Molecular weight expected аpparent193 | 170 kDa (Arabidopsis thaliana)
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Verified reactivityArabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum
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Possible reactivityAmborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandis, Glycine max, Chlorella variabilis, Leucaena glauca, Lotus japonicus, Medicago tribuloides, Mimulus guttatus, Musa malaccensis, Oryza sativa, Panicum italicum, Physcomitrella patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera
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No reactivityNicotiana benthamiana
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Supplementary informationPlease, note that fresh samples will provide better restuls (see image below).
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ReferencesRefer to PubMed
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Scientific contextClathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.
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NotesNone
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Protein numberQ0WNJ6
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TAIR numberAT3G11130
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PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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ConjugationHRP Conjugated
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Gene target
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Short nameClathrin, Clathrin heavy-chain 1 2 (40 µg, -conjugated)
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Techniqueantibody Conjugates
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LabelHRP
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Alternative nameClathrin, Clathrin heavy-epitope 1,2 (40 µg, horseradish peroxidase-coupled)
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