SUMO2, human recombinant

• Catalog number
  4942-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  SUMO-2, Ubiquitin-like protein SMT3B, SMT3 homolog 2, Sentrin-2, HSMT3, SUMO-3, Sentrin2, SUMO2, SMT3H2, MGC117191.
• Alternative_names
  SUMO-2, Ubiquitin-like protein SMT3B, SMT3 homolog 2, Sentrin-2, HSMT3, SUMO-3, Sentrin2, SUMO2, SMT3H2, MGC117191.
• Description
  A substrate for deSUMOylating enzymes.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Typical in vitro concentrations for conjugate formation is 10-50 µM depending on conditions.
• Results
  10-50 µM
• Molecular Weight
  11.6 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  In 50 mM HEPES, pH 8.0, plus 150 mM NaCl, 1 mM DTT.
• Background Information
  SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation. SUMO1 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. Still, unlike ubiquitin which targets proteins for degradation, SUMO-2 is involved in a variety of Cellular processes, for example nuclear transport, transcriptional regulation, apoptosis, and protein stability. The active recombinant SUMO-2 is derived from the precursor pro-SUMO-2 (Accession # NM_006937). Human SUMO-2 shares 44% and 86% identity with SUMO-1 and SUMO-3 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (IκBα, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, transcriptional regulation, apoptosis and protein stability.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  SUMO2  
• Gene symbol
  SUMO2
• Short name
  SUMO2, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  SUMO2, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:11125
• Gene
  SUMO2
• Long gene name
  small ubiquitin like modifier 2
• Synonyms gene
  • SMT3H2
• Synonyms gene name
  • SMT3 (suppressor of mif two 3, yeast) homolog 2
  • SMT3 suppressor of mif two 3 homolog 2 (yeast)
  • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• Synonyms
  • SMT3B
• Locus
  17q25
• Discovery year
  1997-01-29
• Entrez gene record
  6613
• Pubmed identfication
  • 8630065
• RefSeq identity
  • NM_006937
• VEGA ID
  OTTHUMG00000179481

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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