Soluble in water
Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
Laboratory chemicals *For Research Use Only*
To order Biotin-20-dUTP *1 mM in Tris Buffer (pH 7.5)*, please use the Cat. Nr.17018 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Buffering solutions are useful to keep the pH range sable when using this reagent of aat.
-20-dUTP *1 mM in Tris (pH 7.5)*
biotinilated-20-dUTP *1 millimeter in Tris hydroxymethyl aminomethane solution (pH 7.5)*