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Category
Proteins & Peptides
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Long description
When HGF/SF activates c-Met, the first proteins to be activated downstream are Grb2 (growth factor receptor bound protein 2) and Gab 1 (growth factor receptor bound protein 2 associated binder 1). Grb2 in turn may activate a number of kinase pathways, including the pathway from Ras to Raf to Mek and to MAPK(mitogen-activated protein kinase). Gab 1 activates PI3K (phosphoinositide 3 kinase), which activates STAT3 (signal transducer and activator of transcription). c-Met activation also induces activation of beta catenin, a key component of the wnt pathway, which translocates into the nucleus and participates in transcription regulation._x000B__x000B_The HGF/c-Met pathway plays an important role in the development of cancer. First through the activation of key oncogenic pathways (Ras, PI3K/STAT3, beta catenin), secondly through endothelial cell proliferation (neoangiogenesis), thirdly through increased protease production and hence cell dissociation leading to metastasis.
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Antibody come from
Rabbit polyclonal Caspase-4 antibody was raised against a 16 amino acid peptide from the amino-terminus of human Caspase-4.
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Other description
Provided as solution in phosphate buffered saline with 0.02% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
c-Met - Active Enzyme
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Antibody is raised in
Sf9 cells
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Enzyme Kinetic studies, screening inhibitors, selectivity profiling
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Antibody s suited for
Casp-4 (NT) antibody can be used for the detection of Caspase-4 by Western blot at 1 ug/ml. Depending on cell lines or tissues used, other cleavage products may be observed. Antibody can also be used for immunohistochemistry (2-4 µg/ml starting dilution). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases.Cell 2004; 117:561-74_x000B_2) Kuida K, Lippke JA, Ku G, et al. Altered cytokine export and apoptosis in mice deficient in interleukin-1 β converting enzyme. Science 1995; 267:2000-3_x000B_3) Gracie JA, Robertson SE, and McInnes IB. Interleukin-18. J. Leukoc. Biol. 2003; 73:213-224._x000B_4) Kamens J, Paskind M, Hugunin M, et al. Identification and characterization of ICH-2, a novel member of the interleukin-1-converting enzyme family of cysteine proteases.J. Biol. Chem. 1995; 270:15250-6.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
