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Human Recombinant PKM2

Human Recombinant PKM2 is available 1 time from Biovision labs

6372-100 | Human Recombinant PKM2 size: 100 ug | 411.43 USD

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Synonyms Human Recombinant PKM2
Alternative_names Pyruvate kinase, muscle isoform M2, CTHBP, THBP1, p58, M2-PK, Tumor M2-PK, PK2, PK3, PKM.
Description A key enzyme in the glycolytic pathway
Recombinant Yes
Source E. Coli
Purity by SDS-PAGE ≥95%
Assay SDS-PAGE
Biological activity Specific activity: >5.0 unit/mg.
Results >5.0 unit/mg
Unit Definition One unit will form 1.0 µmol of phosphoenolpyruvate to pyruvate per minute at pH 7.5 at 37°C.
Molecular Weight 60.1 kDa (551 aa, 1-531 aa + NT His-Tag)
Storage Temp. -20°C
Shipping gel pack
Shelf Life 12 months
Concentration 1 mg/ml
Appearance Liquid
Physical form description 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
Background Information Pyruvate kinase is a key enzyme in the glycolytic pathway. The M2 isoenzyme of pyruvate kinase is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. The marker is useful for measuring disease activity, sensitivity to chemotherapy and recurrence. PKM2 interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis.
Amino acid sequence MGSSHHHHHH SSGLVPRGSH MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetPKM2
Short name Recombinant PKM2
Technique Recombinant
Species Human
Alternative name H. sapiens recombinant pyruvate kinase, muscle
Alternative technique rec
Alternative to gene target pyruvate kinase, muscle, CTHBP and HEL-S-30 and OIP3 and PK3 and PKM2 and TCB and THBP1, PKM2 and IDBG-20117 and ENSG00000067225 and 5315, poly(A) RNA binding, nuclei, Pkm and IDBG-173276 and ENSMUSG00000032294 and 18746, PKM2 and IDBG-645572 and ENSBTAG00000001601 and 512571
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