Caspase-8, mouse recombinant
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Catalog number1188-100
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PricePlease ask
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Size100 units
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SynonymsCaspase 8
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Alternative_namesCaspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog
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DescriptionA cysteine-aspartic acid protease involved in apoptosis
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Activity Specifications test method5000 units/mg
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Unit DefinitionOne unit of the recombinant caspase-8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Storage Temp-70°C
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Shippinggel pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionLyophilized powder
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Reconstitution InstructionsReconstitute to 1 unit per µl in PBS containing 15% glycerol.
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Background InformationCaspase-8 (also know as FLICE, MASH, Mch5) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-8 also exists in cells as an inactive proenzyme. During apoptosis procaspase-8 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-8 consists of large and small subunits which associate to form the active enzyme. Active caspase-8 has been shown to activate caspase-3 leading to degradation of a variety of cellular target proteins during apoptosis. The recombinant active mouse caspase-8 was expressed in E. coli. The active caspase-8 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Additional descriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolCASP8, CARD8, CASP8AP2
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Short nameCaspase-8, mouse recombinant
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TechniqueRecombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Hostmouse
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SpeciesMouse, Mouses
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Alternative namecaspase-8, mouse Rec.
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Alternative techniquerec, murine
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Gene info
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Identity
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Gene
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Long gene namecaspase 8
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Synonyms gene name
- caspase 8, apoptosis-related cysteine protease
- caspase 8, apoptosis-related cysteine peptidase
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-11-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspases
- Death effector domain containing
- Death inducing signaling complex
- Ripoptosome
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecaspase recruitment domain family member 8
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Synonyms gene name
- caspase recruitment domain family, member 8
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Caspase recruitment domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecaspase 8 associated protein 2
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Synonyms gene name
- CASP8 associated protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-12-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data