100ug-Anti-FLAG Octapeptide (FLAG) Tag Polyclonal Antibody

  • Catalog number
    TAX159Ge01-100ug
  • Price
    Please ask
  • Size
    100ug
  • Organism Species
    Pan-species (General)
  • Source
    Polyclonal antibody preparation
  • Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
  • Buffer Formulation
    PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • Item Name
    FLAG Octapeptide
  • Immunogen
    CPX159Ge21-OVA Conjugated FLAG Octapeptide (FLAG)
  • Image number
    -
  • Species reactivity
    Pan-species
  • Sequence of immunogen
    CPX159Ge21-OVA Conjugated FLAG Octapeptide (FLAG)
  • Aplication
    WB; IHC; ICC; IP.
  • Clonality
    Rabbit polyclonal
  • Concentration
    500ug/ml
  • Alternative Names
    FLAG epitope; FLAG-Tag
  • Applicable Secondary Antibody
    SAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17
  • Delivery condition
    4℃ with ice bags
  • Storage instructions
    Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
  • Conjugation
    Flag
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
  • French translation
    anticorps
  • Gene target
    100ug   Octapeptide   Tag  
  • Short name
    100ug-Anti- Octapeptide ( ) Tag Polyclonal Antibody
  • Technique
    Polyclonal, Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Tags
  • Host
    Rabbit
  • Label
    Flag
  • Alternative name
    100ug-Antibody toFLAG Octapeptide (FLAG) detection labelled polyclonal (antibody to-)
  • Alternative technique
    polyclonals, antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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