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Human Recombinant UCHL5

Human Recombinant UCHL5 is available 1 time from Biovision labs

6359-100 | Human Recombinant UCHL5 size: 100 ug | 502.41 USD

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Synonyms Human Recombinant UCHL5
Alternative_names Ubiquitin carboxyl-terminal hydrolase isozyme L5, UCH37
Description A deubiquitinating enzyme
Recombinant Yes
Source E. Coli
Purity by SDS-PAGE ≥90%
Assay SDS-PAGE
Biological activity Specific activity: >500 pmole/min/µg. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C.
Molecular Weight 39.7 kDa (349 aa, 1-329 aa + NT His Tag)
Storage Temp. -20°C
Shipping gel pack
Shelf Life 12 months
Concentration 0.5 mg/ml
Appearance Liquid
Physical form description 0.5 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 5 mM DTT, 200 mM NaCl, 0.1 mM PMSF, 2 mM EDTA and 30% glycerol.
Background Information UCHL5 is a member of the peptidase C12 family. It is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and is the deubiquitinating enzyme related to the 19S regulatory subunit of the 26S proteasome.
Amino acid sequence MGSSHHHHHH SSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetUCHL5
Short name Recombinant UCHL5
Technique Recombinant
Species Human
Alternative name H. sapiens recombinant ubiquitin carboxyl-terminal hydrolase L5
Alternative technique rec
Alternative to gene target ubiquitin carboxyl-terminal hydrolase L5, UCHL5 and IDBG-105454 and ENSG00000116750 and 51377, proteasome binding, nuclei, Uchl5 and IDBG-197081 and ENSMUSG00000018189 and 56207, UCHL5 and IDBG-629660 and ENSBTAG00000013620 and 282110
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