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Available ordering format
Liquid in PBS pH 8.0, 0.02% sodium azide
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Immunogen
amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.
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Raised in
Hen
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Total IgY
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
Secondary antibodies
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Recommended dilutions for use
1: 5000 (WB)
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Molecular weight expected аpparent
48 kDa
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Verified reactivity
Pseudomonas aeruginosa
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Possible reactivity
Pseudomonas aeruginosa only
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
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References
Anantharajah et al. (2016). Inhibition of the Injectisome and Flagellar Type III Secretion Systems by INP1855 Impairs Pseudomonas aeruginosa Pathogenicity and Inflammasome Activation. J Infect Dis. 2016 Jul 13. pii: jiw295.
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Scientific context
Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.
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Notes
None
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Protein number
Q51449 , PA3841
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TAIR number
Refer to NCBI
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