E. coli MutY Enzyme & Buffer

  • Catalog number
    4000-500-EB
  • Price
    Please ask
  • Size
    5000 units
  • Keep at
    -20° C
  • Assay performance
    The FLARE Sample Prep (slides, lysis solution and low melt agarose) are for use with the FLARE Modules, enzyme and buffers for FLARE, Fragment Length Analysis using Repair Enzymes, detecting DNA damage in single cells using E. coli formamidopyrimidine-DNA glycosylase (Fpg) with single cell gel electrophoresis for DNA damage from mutagens, drugs, Fpg catalyzes the excision of the following forms of DNA damage:opening from 7-methylguanine, including 2,6-diamino-4-hydroxy-5-N-methylformamido-pyrimidine, and 4,6-diamino-5-formamidopyimidine, 8- OHdG, 4-HNA or oxoguaninelesions 5-hydroxycytosine and 5-hydroxyuracil, Aflatoxin-bound imidazole-ring-opened guanine, Imidazole ring opened N-2-aminofluorene-C8-guanine. The FLARE slides are then immersed in an alkali solution, to unwind and denature the DNA strands prior to alkali gel electrophoresis.
  • More advice
    The FLARE Sample Preparation kit contains the necessary comet assay reagents for preparing your samples for incubation with DNA Repair Enzymes to determine the DNA damage occurred by substrate specificity like by Fpg Comet is used to see the tail length, DNA% in the tail, and tail moment. Trevigen FLARE Slides, in FLARE Assay Kits contain low melting agarose and are not used in diagnostic procedures
  • Description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Trevigen. Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
  • Gene target
    MutY  
  • Gene symbol
    MUTYH
  • Short name
    MutY Enzyme &
  • Technique
    Enzyme, enzymes
  • Host
    e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Trevigen supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
  • Species
    E. coli, E. coli
  • Alternative name
    E. coli MutY Enzyme & solution
  • Alternative technique
    enzymes, escherichia
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