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E. coli MutY Enzyme & Buffer

E. coli MutY Enzyme & Buffer is available 1 time from Trevigen labs

4000-500-EB | E. coli MutY Enzyme & Buffer size: 5000 units | 516.42 USD

Catalog number 4000-500-EB
Supplier Trevigen
Price 516.42 USD
Size 5000 units
1. Gene info
Identity 7527
Gene MUTYH
Long gene name mutY DNA glycosylase
Synonyms gene name
  • mutY homolog (E. coli)
Synonyms
  • MYH
GenBank acession
  • U63329
Locus 1p34.1
Discovery year 1997-10-24
Entrez gene record 4595
Pubmed identfication
  • 7823963
  • 10684930
RefSeq identity
  • NM_012222
Classification
  • DNA glycosylases
Havana BLAST/BLAT OTTHUMG00000007682
Locus Specific Databases
  • MUTYH @ LOVD
  • Global Variome shared LOVD
  • LRG_220
Keep at -20° C
Assay performance The FLARE Sample Prep (slides, lysis solution and low melt agarose) are for use with the FLARE Modules, enzyme and buffers for FLARE, Fragment Length Analysis using Repair Enzymes, detecting DNA damage in single cells using E. coli formamidopyrimidine-DNA glycosylase (Fpg) with single cell gel electrophoresis for DNA damage from mutagens, drugs, Fpg catalyzes the excision of the following forms of DNA damage:opening from 7-methylguanine, including 2,6-diamino-4-hydroxy-5-N-methylformamido-pyrimidine, and 4,6-diamino-5-formamidopyimidine, 8- OHdG, 4-HNA or oxoguaninelesions 5-hydroxycytosine and 5-hydroxyuracil, Aflatoxin-bound imidazole-ring-opened guanine, Imidazole ring opened N-2-aminofluorene-C8-guanine. The FLARE slides are then immersed in an alkali solution, to unwind and denature the DNA strands prior to alkali gel electrophoresis.
More advice The FLARE Sample Preparation kit contains the necessary comet assay reagents for preparing your samples for incubation with DNA Repair Enzymes to determine the DNA damage occurred by substrate specificity like by Fpg Comet is used to see the tail length, DNA% in the tail, and tail moment. Trevigen FLARE Slides, in FLARE Assay Kits contain low melting agarose and are not used in diagnostic procedures
Description Buffering solutions are useful to keep the pH range sable when using this reagent of Trevigen.Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
Gene targetMutY
Short name MutY Enzyme
Technique Enzyme
Host e. coli
Species E. coli
Alternative name E. coli MutY Enzyme & solution
Alternative technique enzymes
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MutY Enzyme | Technique alternative | 01011887501
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