-
Alternative name 1
CD34, Clone: ICO-115, Mab anti-Human; frozen/stem cells, IH/flow
-
Alternative name 2
CD34, Clone: ICO-115, Mab antibody-Human; frozen/stem cells, IH/flow
-
Alternative name 3
CD34, Clone: ICO-115, Mab antibody-Homo sapiens; frozen/stem cells, IH/flow
-
Alternative name 4
anti-CD34 Clone: ICO-115 /stem cells
-
Alternative name 5
CD34 Clone: ICO-115 /stem cells
-
Clonality
Monoclonal Antibody
-
Description
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
-
Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
-
Advisory
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
-
Properties
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Test
Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain. Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Latin name
Mus musculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
CD34, SNORA47, SNORD23, RRP15, MAP7, CLIP2, OR4H6P
-
Short name
CD34, Clone: ICO-115, Mouse Monoclonal antibody- ; frozen/stem cells, IH/
-
Technique
Mouse monoclonal, Antibody, frozen, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
-
Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
-
Species
Human, Humans, Mouses
-
Alternative name
CD34 molecule, clonality: ICO-115, Mouse monoclonal (antibody to-)-H. sapiens; -25°C frozen/progenitor cells, IH/flow
-
Alternative technique
antibodies, murine
-
Alternative to gene target
CD34 molecule, CD34 and IDBG-106399 and ENSG00000174059 and 947, sulfate binding, Extracellular, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051
-
Tissue
cells, frozen, stem
-
