Synthetic peptide amino acids 840-857 of rat Shank3
Antibody's other name
AI841104 antibody, DEL22q13.3 antibody, KIAA1650 antibody, Proline rich synapse associated protein 2 antibody, Proline-rich synapse-associated protein 2 antibody, ProSAP2 antibody, PSAP2 antibody, SH3 and multiple ankyrin repeat domains 3 antibody, SH3 and multiple ankyrin repeat domains protein 3 antibody SH3/ankyrin domain gene 3 antibody, SHAN3_HUMAN antibody, Shank postsynaptic density protein antibody, Shank3 antibody, Shank3b antibody, SPANK 2 antibody, SPANK2 antibody
Primary research fields
Neuroscience, Cell Structure, Post-Synaptic Markers
WB, IHC, ICC/IF, IP, AM
Human, Mouse, Rat
WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Protein G Purified
Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Detects ~190kDa. No cross-reactivity against Shank1 or Shank2.
Blue Ice or 4ºC
Antibody certificate of analysis
1 µg/ml of SMC-336 was sufficient for detection of Shank3 in 10 µg COS cell lysate transiently transfected with Shank3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody in cell
Cytoplasm , Cell Junction , Synapse , Postsynaptic Cell Membrane , Postsynaptic Density
See included datasheet or contact our support service
Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins (1). Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Shank proteins are also crucial in receptor tyrosine kinase signaling; specifically, Shank3 can mediate Erk-MAPK and P13K signaling which is crucial for tubule formation (2). Shank3 is also one of the latest genes to be associated with autism. A mutation of a single copy of Shank3 on chromosome 22q13 can result in language and/or social communication disorders (3).
1. Sheng M., and Kim E. (2000) Journal of Cell Science. 113: 1851-1856. 2. Schuetz G., et al. (2004) JCB. 167(5): 645-952. 3. Durand C.M., et al. (2007) Nature Genetics. 39: 25-27.
To be tested
To be tested
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (SMC-336). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (SMC-336) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining. Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (SMC-336). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (SMC-336) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Borderline positive. Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein using Mouse Anti-SHANK3 Monoclonal Antibody, Clone S69-46 (SMC-336). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK3 Monoclonal Antibody (SMC-336) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-SHANK3 Antibody [S69-46] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-336) Mouse Anti-SHANK3 Antibody [S69-46] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-336) Mouse Anti-SHANK3 Antibody [S69-46] used in Western Blot (WB) on Rat brain membrane lysate (SMC-336)
Country of production
Total weight (kg)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus,Rattus norvegicus