EZQuant™ dsDNA Quantitation Kit (Fluorometric)

  • Catalog number
    K900-2000
  • Price
    Please ask
  • Size
    2000 assays
  • Description
    Non-radioactive, Highly sensitive, Specifically detects dsDNA
  • Summary
    • Detection method- Fluorescence (Ex/Em = 480/530 nm) • Application- Quantitation of dsDNA from viral DNA, miniprep DNA, genomic DNA, PCR amplification products etc.
  • Detection Method
    Fluorescence (Ex/Em = 480/530 nm)
  • Applications
    Quantitation of dsDNA from viral DNA, miniprep DNA, genomic DNA, PCR amplification products etc.
  • Sample Type
    dsDNA sample in solution
  • Features Benefits
    • Non-radioactive • Highly sensitive • Specifically detects dsDNA in the presence of ssDNA, RNA, and free nucleotides
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Accurate and sensitive quantification of double stranded DNA (dsDNA) has wide applications. The major disadvantages of traditional absorbance based method for DNA quantitation include (1) lower sensitivity, (2) inability to distinguish between DNA and RNA, (3) interference due to contaminants commonly found in nucleic acid preparations and (4) relatively large contribution of nucleotides and single-stranded nucleic acid to the signal. BioVision’s dsDNA Quantitation Kit is sensitive, non-radioactive and can specifically detect dsDNA in the presence of ssDNA, RNA, and free nucleotides. The assay is based on the fluorescent dsDNA-binding dye that specifically binds to dsDNA to generate fluorescence (Ex/Em = 480/530 nm), which enables the sensitive quantitation of small amounts of dsDNA (as low as 40 pg). This highly sensitive assay is ready as supplied for 96-well plate format and can be adapted to 384-well plate.
  • Gene target
  • Short name
    EZQuant™ dsDNA Quantitation Kit (Fluorometric)
  • Alternative name
    EZQuant™ dsDNA Quantitation reagent (fluorescence)
  • Alternative technique
    kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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