Deproteinizing Sample Preparation Kit

  • Catalog number
    K808-200
  • Price
    Please ask
  • Size
    200 assays
  • Description
    Simple Kit to deproteinize samples
  • Summary
    • Species reactivity- Mammalian • Application- Unique tool to prepare samples for the analysis of a variety of small molecules, proteins are precipitated, excess PCA is removed, samples are neutralized.
  • Sample Type
    Cell and tissue culture homogenates (eukaryotic cells), serum, plasma and high protein samples
  • Features Benefits
    • Simple procedure; takes ~ 20 minutes • Fast and convenient • Samples prepared using this kit can be directly used in a wide variety of small molecule bioassays.
  • Storage Conditions
    RT
  • Shipping Conditions
    RT
  • Shelf life
    12 months
  • Background
    The presence of protein and various enzyme activities frequently interferes with the analysis of small molecules in biological samples. Many bioassays require removal of proteins from samples prior to analysis. Among the deproteinization protocols developed over the last half century, perchloric acid (PCA) precipitation has been extensively used in many different sample preparation procedures, since not only does it remove most of the protein present but it also functions to stabilize many of the small molecule analytes. PCA deproteinization methods have been successfully used in the preparation samples prior to quantitation of an array of small molecules, including glycogen, ATP, cAMP, glutathione, antioxidants, etc.The newly designed Deproteinizing Sample Preparation Kit utilizes a PCA precipitation method, providing a unique tool to prepare samples for the analysis of a variety of small molecules. Using the kit, proteins are precipitated, excess PCA is removed, samples are neutralized. Samples prepared using this kit can be directly used in a wide variety of bioassays. The method is easy, convenient, and can be used for the preparation of a large number of samples.
  • Gene target
  • Short name
    Deproteinizing Sample Preparation Kit
  • Technique
    Sample, samples
  • Alternative name
    Deproteinizing Sample Preparation reagent
  • Alternative technique
    samples, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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