-
Available ordering format
Lyophilized
-
Immunogen
KLH-conjugated peptide derived from the sequence of human glycogen debranching enzyme P35573
-
Raised in
Rabbit
-
Clonality
Polyclonal
-
Clone
Polyclonal
-
Purification
Affinity purified serum
-
How to reconstitute
For reconstitution add 200 µl of sterile water
-
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
-
Verified applications
western blot (WB), immunoprecipitation (IP)
-
Connected products
AS09 455 | anti-glycogen phosphorylaseSecondary antibodies
-
Recommended dilutions for use
1: 2000 (WB), 5 µg (IP)
-
Molecular weight expected аpparent
175 kDa
-
Verified reactivity
human, mouse, rabbit,rat
-
Possible reactivity
dog
-
No reactivity
no confirmed exceptions from predicted reactivity known in the moment
-
Supplementary information
samples used to test this antibody were: muscle and liver homogenates, purified glycogen
-
References
Oldenburg et al. (2016). CD44 and RHAMM are essential for rapid growth of bladder cancer driven by loss of Glycogen Debranching Enzyme (AGL). BMC Cancer. 2016 Sep 5;16:713. doi: 10.1186/s12885-016-2756-5.Pagliarani et al. (2014). Glycogen storage disease type III: A novel Agl knockout mouse model. Biochim Biophys Acta. 2014 Aug 1. pii: S0925-4439(14)00250-6. doi: 10.1016/j.bbadis.2014.07.029.
-
Scientific context
Synonymes:Glycogen debrancher, 4-alpha-glucanotransferase (EC=2.4.1.25), Oligo-1,4-1,4-glucantransferase, amylo-alpha-1,6-glucosidase, amylo-1,6-glucosidase (EC=3.2.1.33), Dextrin 6-alpha-D-glucosidase,amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
-
Notes
This antibody can detect GDE in crude tissue homogenate in both, liver and muscle tissue and is able to immunoprecipiate GDE.Antibody will work as an excellent marker for glycogen. In tissues with no glycogen extression there will be no signal using this antibody.
-
Protein number
P35573
-
TAIR number
Refer to NCBI
-
-
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
