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VeriScript Reverse Transcriptase

VeriScript Reverse Transcriptase is available 1 time from Biovision labs

9224-1000 | VeriScript Reverse Transcriptase size: 1000 UN | 155.44 USD

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Other name Verscript
Aa sequence N/A
Unit One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into acid insoluble material in 10 minutes at 37°C using poly(rA)-oligo(dT)18 as template-primer.
Reesults N/A
Molecular weight N/A
Source Recombinant
Storage condition -20°C
Level of endotoxin N/A
Binding ability N/A
Tested applications SDS-PAGE, N/A
Notes For Research Use Only! Not to be used in humans
Recombinant Yes
Tested activity 200 unit/μl
Purification ≥95%
Background VeriScript Reverse Transcriptase is an engineered version of M-MLV Reverse Transcriptase with reduced RNase H activity and increased thermostability, allowing for first-strand cDNA synthesis with more full length cDNAs up to 12.5 kb. This DNA polymerase can synthesize complementary DNA from single-stranded DNA, RNA, or a RNA:DNA hybrid.
Before use Centrifuge the vial prior to opening.
Shipping under Gel pack
Concentration N/A
Physical properties Recombinant VeriScript Reverse Transcriptase in 20mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01% Igepal CA-630, and 50% Glycerol.
Biological activity N/A
Physical appearance Liquid
Synonym Verscript
How to reconstitute N/A
Description Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
Goup reverse transcription
Short name VeriScript Reverse Transcriptase
Technique reverse
Alternative name VeriScript Reverse Transcriptase
Alternative technique transctription
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