Human Whole Blood Polymorphonuclear Cell Isolation Kit

• Catalog number
  K483-30
• Price
  Please ask
• Size
  30 isolations

• Description
  Simple, one step assay to isolate PMN
• Summary
  • Isolation of PMNs from human whole blood. • Determination of cell viability and effectively removes PBMC cells.
• Detection Method
  Not applicable
• Species Reactivity
  Human
• Applications
  • Isolation of PMNs from human whole blood. • Determination of cell viability and effectively removes PBMC cells.
• Sample Type
  Human whole blood (collected within 12 hr)
• Features Benefits
  • PMN high yields (≥5 x106 cells/ml) • By using the Viability Stain, it was proven that 99% of the cells are viable and the isolated fraction contains low red blood cells (≤ 3%)
• Storage Conditions
  4 °C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Polymorphonuclear cells (PMN) have a multilobed nucleus and include the following cells types: mast cells, neutrophils, eosinophils and basophils. Upon isolation, these cells can be used for migration assays and determination of cytokine/chemokine production in response to pharmacological compounds. BioVision’s PMN Cell Isolation Kit is unique in the market because it enables the user to isolate PMNs, assess viability of the obtained cell fractions and also determine purity with three simple, easy-to-use reagents! High yields, or 60-80% of total PMNs have been obtained yielding ≥ 5 x106 cells per ml of human whole blood. Purity of the cell fraction is assessed with giemsa stain (included). BioVision’s kit use results in highly enriched (>95%) cell fraction of polymorphonuclear cells with less than 3% red blood cell counts. More than 99% of the cells are viable, as determined by the Viability Stain
• Data Sheet Link
• Additional description
  Blood is slightly basic and contains proteins, hemoglobin, albumins in its serum. Biovision supplies this blood related product in 1. For cells, cell lines and tissues in culture till half confluency.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

• Gene target
  Whole   Polymorphonuclear   Isolation   Kit  
• Short name
  Whole Blood Polymorphonuclear Isolation Kit
• Technique
  blood, blood samples
• Species
  Human, Humans
• Alternative name
  H. sapiens Whole Blood Polymorphonuclear cellular purification reagent
• Alternative technique
  sera, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  blood, cell, whole

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