Sphingosine Kinase 1 (Full Length) with N-Terminal HIS tag - Active Enzyme

  • Catalog number
    X1708E
  • Price
    Please ask
  • Size
    10 μg
  • Category
    Proteins & Peptides
  • Long description
    Sphingosine Kinase 1 (Sphk1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
  • Antibody come from
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a cytoskeletal preparation of OTN-11 ovarian carcinoma cells and SP2/0-Ag14 mouse myeloma cells.
  • Other description
    Provided as solution in phosphate buffered saline with 0.08% sodium azide.
  • Clone
    not specified
  • Antigen antibody binding interaction
    Sphingosine Kinase 1 (Full Length) with N-Terminal HIS tag - Active Enzyme
  • Antibody is raised in
    Sf9 cells
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    No Data Available
  • Application
    Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
  • Antibody s suited for
    Antibody can be used for immunohistochemistry on frozen or paraffin-embedded tissue, immunocytochemistry, immunoblotting (1:100-1:1000) and flow cytometry (1:100-1:200). Frozen sections require fixation in methanol. Paraffin-embedded sections require pretreatment with 0.1% pronase/0.1% trypsin in PBS or 0.5% pepsin in 0.01N HCl. Optimal concentration should be evaluated by serial dilutions.
  • Storage
    -80ºC
  • Relevant references
    1. Ramaekers, F., et al. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55._x000B__x000B_2. van Niekerk, C. C., et al. (1991). Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues, J Pathol 165, 145-52._x000B__x000B_3. van Niekerk, C. C., et al. (1991). Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas, Am J Pathol 138, 455- 63._x000B__x000B_4. van de Molengraft, F. J., et al. (1993). OV-TL 12/30 (keratin 7 antibody) is a marker of_x000B_glandular differentiation in lung cancer, Histopathology 22, 35-8._x000B__x000B_5. van Niekerk, C. C., et al. (1997). Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies, Cancer Detect Prev 21, 247-57._x000B__x000B_6. Torenbeek, R., et al. (1998). Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma, Histopathology 32, 20-7.
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
  • Conjugation
    histidine
  • Gene target
    Sphingosine   Kinase   Full   Length   with   HIS   tag   Active  
  • Gene symbol
    HAL, VIS1, AKR1B10, TRH-GTG1-1, TRH-GTG1-3, TRH-GTG2-1, TRH-GTG3-1, TRH-GTG1-2, TRH-GTG1-4, TRH-GTG1-5
  • Short name
    Sphingosine Kinase 1 (Full Length) with N-Terminal HIS tag - Active Enzyme
  • Technique
    Enzyme, enzymes, Tags
  • Isotype
    N/A
  • Label
    unlabeled
  • Alternative name
    Sphingosine phosphorylation catalyst 1 (complete Length) including N-Terminal histidine detection labelled - functionnal Enzyme
  • Alternative technique
    enzymes
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 4 (anticodon GUG)
    • transfer RNA-His (GTG) 1-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-21
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-3
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 9 (anticodon GUG)
    • transfer RNA-His (GTG) 1-3
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (GTG) 2-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 11 (anticodon GUG)
    • transfer RNA-His (GTG) 2-1
  • Synonyms
  • GenBank acession
  • Locus
    1
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Low confidence cytoplasmic transfer RNAs
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-2
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 10 (anticodon GUG)
    • transfer RNA-His (GTG) 1-2
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-4
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 7 (anticodon GUG)
    • transfer RNA-His (GTG) 1-4
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-His (anticodon GTG) 1-5
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA histidine 3 (anticodon GUG)
    • transfer RNA-His (GTG) 1-8
    • transfer RNA-His (GTG) 1-5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-09-03
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
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