Sphingosine Kinase 1 (Sphk1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a cytoskeletal preparation of OTN-11 ovarian carcinoma cells and SP2/0-Ag14 mouse myeloma cells.
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
Antigen-antibody binding interaction
Sphingosine Kinase 1 (Full Length) with N-Terminal HIS tag - Active Enzyme
Antibody is raised in
Antibody's reacts with
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Antibody's suited for
Antibody can be used for immunohistochemistry on frozen or paraffin-embedded tissue, immunocytochemistry, immunoblotting (1:100-1:1000) and flow cytometry (1:100-1:200). Frozen sections require fixation in methanol. Paraffin-embedded sections require pretreatment with 0.1% pronase/0.1% trypsin in PBS or 0.5% pepsin in 0.01N HCl. Optimal concentration should be evaluated by serial dilutions.
1. Ramaekers, F., et al. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55._x000B__x000B_2. van Niekerk, C. C., et al. (1991). Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues, J Pathol 165, 145-52._x000B__x000B_3. van Niekerk, C. C., et al. (1991). Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas, Am J Pathol 138, 455- 63._x000B__x000B_4. van de Molengraft, F. J., et al. (1993). OV-TL 12/30 (keratin 7 antibody) is a marker of_x000B_glandular differentiation in lung cancer, Histopathology 22, 35-8._x000B__x000B_5. van Niekerk, C. C., et al. (1997). Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies, Cancer Detect Prev 21, 247-57._x000B__x000B_6. Torenbeek, R., et al. (1998). Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma, Histopathology 32, 20-7.
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.