Sphingosine Kinase 1 (Full Length) with N-Terminal HIS tag - Active Enzyme
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Catalog numberX1708E
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PricePlease ask
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Size10 μg
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CategoryProteins & Peptides
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Long descriptionSphingosine Kinase 1 (Sphk1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
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Antibody come fromHybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a cytoskeletal preparation of OTN-11 ovarian carcinoma cells and SP2/0-Ag14 mouse myeloma cells.
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Other descriptionProvided as solution in phosphate buffered saline with 0.08% sodium azide.
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Clonenot specified
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Antigen antibody binding interactionSphingosine Kinase 1 (Full Length) with N-Terminal HIS tag - Active Enzyme
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Antibody is raised inSf9 cells
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Antibody s reacts withHuman
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityNo Data Available
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ApplicationUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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Antibody s suited forAntibody can be used for immunohistochemistry on frozen or paraffin-embedded tissue, immunocytochemistry, immunoblotting (1:100-1:1000) and flow cytometry (1:100-1:200). Frozen sections require fixation in methanol. Paraffin-embedded sections require pretreatment with 0.1% pronase/0.1% trypsin in PBS or 0.5% pepsin in 0.01N HCl. Optimal concentration should be evaluated by serial dilutions.
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Storage-80ºC
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Relevant references1. Ramaekers, F., et al. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55._x000B__x000B_2. van Niekerk, C. C., et al. (1991). Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues, J Pathol 165, 145-52._x000B__x000B_3. van Niekerk, C. C., et al. (1991). Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas, Am J Pathol 138, 455- 63._x000B__x000B_4. van de Molengraft, F. J., et al. (1993). OV-TL 12/30 (keratin 7 antibody) is a marker of_x000B_glandular differentiation in lung cancer, Histopathology 22, 35-8._x000B__x000B_5. van Niekerk, C. C., et al. (1997). Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies, Cancer Detect Prev 21, 247-57._x000B__x000B_6. Torenbeek, R., et al. (1998). Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma, Histopathology 32, 20-7.
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionEnzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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Conjugationhistidine
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Gene target
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Gene symbolHAL, VIS1, AKR1B10, TRH-GTG1-1, TRH-GTG1-3, TRH-GTG2-1, TRH-GTG3-1, TRH-GTG1-2, TRH-GTG1-4, TRH-GTG1-5
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Short nameSphingosine Kinase 1 (Full Length) with N-Terminal HIS tag - Active Enzyme
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TechniqueEnzyme, enzymes, Tags
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IsotypeN/A
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Labelunlabeled
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Alternative nameSphingosine phosphorylation catalyst 1 (complete Length) including N-Terminal histidine detection labelled - functionnal Enzyme
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Alternative techniqueenzymes
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Gene info
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Identity
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Gene
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Long gene namehistidine ammonia-lyase
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Synonyms gene
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Locus
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Discovery year1988-08-31
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameviral integration site 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-11-25
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene namealdo-keto reductase family 1 member B10
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Synonyms gene
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Synonyms gene name
- aldo-keto reductase family 1, member B10 (aldose reductase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-08-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Aldo-keto reductases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-1
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 4 (anticodon GUG)
- transfer RNA-His (GTG) 1-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-21
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-3
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 9 (anticodon GUG)
- transfer RNA-His (GTG) 1-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (GTG) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 11 (anticodon GUG)
- transfer RNA-His (GTG) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Low confidence cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 3-1
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 8 (anticodon GUG)
- transfer RNA-His (GTG) 3-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-2
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 10 (anticodon GUG)
- transfer RNA-His (GTG) 1-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-4
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 7 (anticodon GUG)
- transfer RNA-His (GTG) 1-4
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-His (anticodon GTG) 1-5
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Synonyms gene
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Synonyms gene name
- transfer RNA histidine 3 (anticodon GUG)
- transfer RNA-His (GTG) 1-8
- transfer RNA-His (GTG) 1-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-09-03
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs