-
Category
Proteins & Peptides
-
Long description
Human Sirtuin 1 (GenBank Accession No. NM_012238), amino acids 193-741 withN-terminal GST tag, MW=87.2 kDa, expressed in E.coli expression system._x000B__x000B_Sirtuin 1 is a NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates Lys-382 of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. Inhibited by nicotinamide. Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'- tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene),Isoliquiritigenin(4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'- pentahydroxyfla.Ivone). 3
-
Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the N-terminus of the human MMP9 protein and mouse myeloma Ag8563 cells. Sequence common in rabbit, dog and pig
-
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
-
Clone
not specified
-
Antigen antibody binding interaction
Sirtuin 1 (193-741) - Active Enzyme
-
Antibody is raised in
E.coli
-
Antibody s reacts with
Human
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
Enyzme activity assay standard
-
Antibody s suited for
Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry (1-5 µg/ml). Antibody is specific for the activated form of MMP9 only. Optimal concentration should be evaluated by serial dilutions.
-
Storage
-80ºC
-
Relevant references
1. Wang, L. et al. (2006). Matrix metalloproteinase 2 (MMP2) and MMP9 secreted by erythropoietin-activated endothelial cells promote neural progenitor cell migration. J. Neurosci. 26(22);5996-6003_x000B__x000B_2. Solmiari, S.B., et al. (2006). Circulating MMP2 and MMP9 in breast cancer -- potential role in classification of patients into low risk, high risk, benign disease and breast cancer categories. Int. J. Cancer. 119(6);1403-1411_x000B__x000B_3. Chen, X., et al. (2005). Increased plasma MMP9 in integrin alpha1-null mice enhances lung metastasis of colon carcinoma cells. Int. J. Cancer. 116(1);52-61
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
