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E coli Fpg Enzyme Buffer

E coli Fpg Enzyme Buffer is available 2 times from Trevigen labs

4040-100-EB | E. coli Fpg Enzyme & Buffer size: 500 units | 512.74 USD

Catalog number 4040-100-EB
Supplier Trevigen
Price 512.74 USD
Size 500 units

4040-500-EB | E. coli Fpg Enzyme & Buffer size: 2500 units | 1,145.29 USD

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Keep at -20° C
Assay performance The FLARE Sample Prep (slides, lysis solution and low melt agarose) are for use with the FLARE Modules, enzyme and buffers for FLARE, Fragment Length Analysis using Repair Enzymes, detecting DNA damage in single cells using E. coli formamidopyrimidine-DNA glycosylase (Fpg) with single cell gel electrophoresis for DNA damage from mutagens, drugs, Fpg catalyzes the excision of the following forms of DNA damage:opening from 7-methylguanine, including 2,6-diamino-4-hydroxy-5-N-methylformamido-pyrimidine, and 4,6-diamino-5-formamidopyimidine, 8- OHdG, 4-HNA or oxoguaninelesions 5-hydroxycytosine and 5-hydroxyuracil, Aflatoxin-bound imidazole-ring-opened guanine, Imidazole ring opened N-2-aminofluorene-C8-guanine. The FLARE slides are then immersed in an alkali solution, to unwind and denature the DNA strands prior to alkali gel electrophoresis.
More advice The FLARE Sample Preparation kit contains the necessary comet assay reagents for preparing your samples for incubation with DNA Repair Enzymes to determine the DNA damage occurred by substrate specificity like by Fpg Comet is used to see the tail length, DNA% in the tail, and tail moment. Trevigen FLARE Slides, in FLARE Assay Kits contain low melting agarose and are not used in diagnostic procedures
Description Buffering solutions are useful to keep the pH range sable when using this reagent of Trevigen.Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
Gene targetFpg
Short name Fpg Enzyme
Technique Enzyme
Host e. coli
Species E. coli
Alternative name E. coli Fpg Enzyme & solution
Alternative technique enzymes
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