CD3E, human recombinant

• Catalog number
  7342-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE
• Alternative_names
  T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE
• Description
  Plays a role in coupling antigen recognition to several intracellular signal-transduction pathways
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Molecular Weight
  14.1 kDa (127 aa, 23-126 aa + His Tag), confirmed by MALDI-TOF.
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, and 10% glycerol.
• Background Information
  CD3E is the CD3-epsilonpolypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. Recombinant human CD3E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MGSDGNEEMG GITQTPYKVS ISGTTVILTC PQYPGSEILW QHNDKNIGGD EDDKNIGSDE DHLSLKEFSE LEQSGYYVCY PRGSKPEDAN FYLYLRARVC ENCMEMD
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CD3E  
• Gene symbol
  CD3E
• Short name
  CD3E, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  CD3e molecule, epsilon (CD3-TCR complex), H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  CD3e molecule, epsilon (CD3-TCR complex), IMD18 and T3E and TCRE, CD3E and IDBG-73617 and ENSG00000198851 and 916, protein heterodimerization activity, Cell surfaces, Cd3e and IDBG-159085 and ENSMUSG00000032093 and 12501, CD3E and IDBG-631260 and ENSBTAG00000015710 and 281054

Gene info

• Identity
  HGNC:1674
• Gene
  CD3E
• Long gene name
  CD3e molecule
• Synonyms gene name
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
• GenBank acession
  • X03884
• Locus
  11q23.3
• Discovery year
  1986-01-01
• Entrez gene record
  916
• RefSeq identity
  • NM_000733
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000166968
• Locus Specific Databases
  • CD3Ebase: Mutation registry for autosomal recessive CD3epsilon immunodeficiency
  • LRG_38

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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