Rabbit, Anti-FLAG Octapeptide (FLAG) Tag Polyclonal Antibody-Polyclonal antibody
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Catalog number
TAX159Ge01-1mg
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Price
Please ask
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Size
1mg
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Organism Species
Pan-species (General)
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Source
Polyclonal antibody preparation
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Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
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Item Name
FLAG Octapeptide
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Immunogen
CPX159Ge21-OVA Conjugated FLAG Octapeptide (FLAG)
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Image number
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Species reactivity
Pan-species
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Sequence of immunogen
CPX159Ge21-OVA Conjugated FLAG Octapeptide (FLAG)
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Aplication
WB; IHC; ICC; IP.
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Clonality
Rabbit polyclonal
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Concentration
500ug/ml
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Alternative Names
FLAG epitope; FLAG-Tag
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Applicable Secondary Antibody
SAA544Mu08, SAA544Mu09, SAA544Mu07, SAA544Mu19, SAA544Mu18, SAA544Mu17
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Delivery condition
4℃ with ice bags
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Storage instructions
Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by Cloud Clone Corp. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cloud Clone Corp adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Rabbit, Anti- Octapeptide ( ) Tag Polyclonal Antibody-Polyclonal antibody
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Technique
Polyclonal, Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope. Tags
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Host
Rabbit, Rabbits
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Label
Flag
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Alternative name
production species: rabbit, Antibody toFLAG Octapeptide (FLAG) detection labelled polyclonal (antibody to-)-polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies, rabbit-anti
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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