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Mouse TIMP 2 PicoKine ELISA Kit

Mouse TIMP 2 PicoKine ELISA Kit is available 2 times from Boster labs

EK0955 | Mouse TIMP-2 PicoKine ELISA Kit size: 1x96 well plate | 631.24 USD

Catalog number EK0955
Supplier boster
Price 631.24 USD
Size 1x96 well plate
1. Gene info
Identity 11821
Gene TIMP2
Long gene name TIMP metallopeptidase inhibitor 2
Synonyms gene name
  • tissue inhibitor of metalloproteinase 2
Synonyms
  • CSC-21K
Locus 17q25.3
Discovery year 1992-06-18
Entrez gene record 7077
Pubmed identfication
  • 1427908
RefSeq identity
  • NM_003255
Classification
  • Tissue inhibitor of metallopeptidases
Havana BLAST/BLAT OTTHUMG00000154517
Product images
    #Mouse#.jpg

EK0955 | Mouse TIMP-2 PicoKine ELISA Kit size: 96wells/kit, with removable strips. | 588.49 USD

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ELISA's application For quantitative measurement of mouse TIMP-2 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
Gene tested murine TIMP-2
ELISA standard curve Concentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.018 + 0.054 + 0.105 + 0.189 + 0.458 + 0.759 + 1.468 + 2.267
ELISA kit detection limits 156pg/ml to 10000pg/ml
ELISA's sensitivity< 10pg/ml
Target of this ELISA Natural and recombinant mouse TIMP-2
Cross reaction the ELISA does not cross react with any other proteins except from closely related species
ELISA's precision parameters intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.24;3.85;6.85;1.32;3.96;6.88 ELISA standard deviations 0.056;0.146;0.219;0.088;0.245;0.516 CV(%);4.5;3.8;3.2;6.7;6.2;7.5
ELISA kit description GENTAUR's mouse TIMP-2 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for TIMP-2 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, C27-P220) and your samples are measured with a biotin conjugated polyclonal from goat specific for TIMP-2 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse TIMP-2 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
Detector and capure antibodies A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
The ELISA contains Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse TIMP-2 antibodies;1 ST0000-10;Lyophilized recombinant mouse TIMP-2 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse TIMP-2 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Anti-coagulation heparin or EDTA
How the elisa works Sample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse TIMP-2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to the ELISA plate. Keep in mind that each mouse TIMP-2 standard solution and each sample be measured in duplicate.
Minutes of TMB incubation 15-20
More gene info TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.
Antigen NSO, C27-P220
Keep at Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Uniprot Metalloproteinase inhibitor 2
Gene name TIMP metallopeptidase inhibitor 2
Alternative CSC 21K;CSC21K;D11Bwg1104e;DDC8;Metalloproteinase inhibitor 2;Metalloproteinase inhibitor 2 precursor;MGC105282;OTTMUSP00000004170;OTTMUSP00000004171;RP23-394O9.2;TIMP 2;TIMP metallopeptidase inhibitor 2;Tissue inhibitor of metalloproteinase 2;Tissue inhibitor of metalloproteinases 2
Gen protein identity P25785
Entrez nr. 21858
Gene Name Timp2
Assay range 156pg/ml-10,000pg/ml
Immunogen Expression system for standard: NSO; Immunogen sequence: C27-P220
Storage & Transport Conditions Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Cross-reactivity There is no detectable cross-reactivity with other relevant proteins.
Kit Components 10ng/tube
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Applications ELISA
Reactivity Mouse
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name Mus musculus
Gene targetTIMP-2 PicoKine
Short name Mouse TIMP-2 PicoKine ELISA Kit
Technique ELISA kit, ELISA, Mouse
Host mouse
Species Mouse
Alternative name Mouse TIMP-2 PicoKine Enzyme-linked immunosorbent assay reagent
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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