EZExtract™ Core Histone Isolation Kit

• Catalog number
  K234-100
• Price
  Please ask
• Size
  100 extractions

• Description
  Simple method to isolate histones
• Summary
  Applications: • Convenient and fast isolation of histones from mammalian tissues • Protein profiling, post-translational modification and epigenetic analyses • Protein profiling of histones by SDS-PAGE and Western blot
• Applications
  • Isolation of intact functional core histones from tissues and cultured cells • Protein profiling, post-translational modification and epigenetic analyses • Protein profiling of Histones by SDS-PAGE and Western blot
• Sample Type
  • Mammalian glands and soft tissues such as liver, spleen, lungs etc. • Cultured eukaryotic cell lines
• Features Benefits
  • Simple & convenient protocol • The kit provides unique formulations of ready-to-use buffers and reagents to isolate histones with minimal sample preparation
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Histones are small alkaline proteins that interact with eukaryotic DNA to form the fundamental subunit of chromatin known as the nucleosome. The core histones H2A, H2B, H3 and H4 form the central spooling unit of the nucleosome; with two copies of each comprising a histone octamer that interacts with the negatively charged DNA. They enable an ordered compaction of the DNA, as well as spatiotemporal control of gene expression and genomic replication throughout mitosis. Post-translational modification of this core complex results in structural changes within the chromatin and epigenetic control of gene expression. BioVision’s EZExtract™ Core Histone Isolation Kit offers an optimized suite of reagents to rapidly extract the total core histones while preserving acetylation from tissues and cultured cells for biochemical and epigenetic analyses. One hundred standard extractions at a scale of 107 cells or 100 mg tissues can be performed with the reagents in this kit. Yield of the total histone proteins is approximately 0.4 mg per 107 cells or 100 mg of tissue
• Data Sheet Link

• Gene target
  EZExtract™   Core   Histone   Isolation   Kit  
• Short name
  EZExtract™ Core Histone Isolation Kit
• Alternative name
  EZExtract™ Core Histone purification reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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