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Human Methylcrotonoyl carboxylase ELISA Kit 96 well plate

Human Methylcrotonoyl carboxylase ELISA Kit 96 well plate is available 6 times from Bioma labs

EKU09175 | Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit, 96 well plate size: 5x96T | 4,045.33 USD

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EKU09175 | Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit, 96 well plate size: 96T | 962.85 USD

Verified reactivity Homo sapiens (Human)
Detection limits 0.312-20 ng/mL
Sensitivity limit 0,141 ng/mL
Samples to be analyzed Serum, plasma, tissue homogenates and other biological fluids.
Assay duration 3 hours
Assay class Sandwich
Protein number Q9HCC0
Gene number 64087
Gene name Methylcrotonoyl Coenzyme A Carboxylase 2
Alternate gene name MCCB; 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta; 3-methylcrotonyl-CoA carboxylase 2
Use for 8 months
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). No significant cross-reactivity or interference between Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) and analogues was observed.
Assay principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research main area Enzyme & Kinase;
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Gene targetMethylcrotonoyl Coenzyme Carboxylase MCCC2
Short name Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit, 96
Alternative name H. sapiens Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative technique enzymes
MeSH Data
Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
Product images
    #Human#.jpg

EKU09175 | Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit, 96 well plate size: 10x96T | 7,309.27 USD

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EKC34604 | Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit, 96 well plate size: 5x96T | 3,534.99 USD

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EKC34604 | Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit, 96 well plate size: 10x96T | 6,578.91 USD

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EKC34604 | Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit, 96 well plate size: 96T | 926.56 USD

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Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Estimated production time 7-11 business days
Shipping requirements Blue ice
ELISA detection Colorimetric
Alternate protein number Please refer to SwissProt
Protocol Please see ELISA's datasheet, otherwise contact us
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Notes For research use only. Not for diagnostic procedures.
Verified applications ELISA
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species Human
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA
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  • Methylcrotonoyl
  • Coenzyme
  • Carboxylase
  • MCCC2
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