pVision-GFP-C Vector

• Catalog number
  9998-20
• Price
  Please ask
• Size
  20 μg

• Description
  An eukaryotic expression vector encoding green fluorescent protein. pVisionGFP-C1 vector is a mammalian expression vector encoding green fluorescent protein, VisionGFP. pVisionGFP-C vector is designed to generate fusions to VisionGFP C-terminus for expression, localization and cellular dynamics studies or to express VisionGFP in eukaryotic (mammalian) cells. pVisionGFP-C vector carries synthetic version of the VisionGFP gene which codon usage is humanized, i.e. optimized for high expression in mammalian cells. pVisionGFP-C vector backbone contains immediate early promoter of cytomegalovirus (PCMV IE) for protein expression, SV40 origin for replication in mammalian cells, pUC origin of replication for propagation in E. coli, and f1 origin for single-stranded DNA production. SV40 early promoter provides neomycin resistance gene expression to select stably transfected eukaryotic cells using G418. Bacterial promoter (P) provides kanamycin resistance gene expression in E. coli. To increase VisionGFP mRNA translation efficiency, Kozak consensus translation initiation site is generated upstream of VisionGFP coding sequence. Multiple cloning site (MCS) is located VisionGFP coding sequence and SV40 polyadenylation signal (SV40 poly A).
• Product Highlights
  • Amount - 20 µg • Application: C-terminal mammalian expression vector encoding humanized TagGFP and allowing TagGFP expression and generation of fusions to the TagGFP C-terminus. Generation of VisionGFP-tagged fusions A localization signal or a gene of interest should be cloned into MCS of the vector. It will be expressed as a fusion to VisionGFP C-terminus when inserted in the same reading frame as VisionGFP and no in-frame stop codons are present. VisionGFP-tagged fusions retain fluorescent properties of the native protein allowing fusion localization in vivo. Unmodified pVisionGFP-C vector will express VisionGFP when transfected into eukaryotic (mammalian) cells. Expression in Mammalian Cells pVisionGFP-C can be transfected into mammalian cells by any known transfection method. CMV promoter provides strong, constitutive expression of VisionGFP or VisionGFP-tagged fusions in many cell types. If required, stable transformants can be selected using G418. Propagation in E. coli -Suitable host strains: DH5alpha, HB101, and other general purpose strains. Single-stranded DNA production requires a host containing an F plasmid such as JM109 or XL1-Blue. - Selectable marker: plasmid confers resistance to kanamycin (30 µg/ml) to E. coli hosts. -E. coli replication origin: pUC - Copy number: ~500 -Plasmid incompatibility group: pMB1/ColE1
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.

• Gene target
  pVision-GFP-C   Vector  
• Gene symbol
  SMC4, MYB, PCK1, SFTPC, VCX3B, MAF, RFX1, EGR4, P4HA2
• Short name
  pVision-GFP-C Vector
• Technique
  Vectors
• Label
  gfp
• Alternative name
  pVision-Green Fluorescent-C integrating Desoxyribonucleic acid sequence

Gene info

• Identity
  HGNC:14013
• Gene
  SMC4
• Long gene name
  structural maintenance of chromosomes 4
• Synonyms gene
  • SMC4L1
• Synonyms gene name
  • SMC4 (structural maintenance of chromosomes 4, yeast)-like 1
  • SMC4 structural maintenance of chromosomes 4-like 1 (yeast)
• Synonyms
  • hCAP-C
  • CAP-C
• GenBank acession
  • AF092564
• Locus
  3q25.33
• Discovery year
  2001-02-22
• Entrez gene record
  10051
• Pubmed identfication
  • 9789013
  • 10319587
• Classification
  • Structural maintenance of chromosomes proteins
  • Condensin I subunits
  • Condensin II subunits
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000159006

Gene info

• Identity
  HGNC:7545
• Gene
  MYB
• Long gene name
  MYB proto-oncogene, transcription factor
• Synonyms gene name
  • v-myb avian myeloblastosis viral oncogene homolog
• Synonyms
  • c-myb
• Locus
  6q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  4602
• Pubmed identfication
  • 17599807
• Classification
  • Myb/SANT domain containing
• VEGA ID
  OTTHUMG00000015629
• Locus Specific Databases
  • The Globin Gene Server

Gene info

• Identity
  HGNC:8724
• Gene
  PCK1
• Long gene name
  phosphoenolpyruvate carboxykinase 1
• Synonyms gene name
  • phosphoenolpyruvate carboxykinase 1 (soluble)
• Synonyms
  • PEPCK-C
• Locus
  20q13.31
• Discovery year
  1990-09-10
• Entrez gene record
  5105
• Pubmed identfication
  • 1492743
• VEGA ID
  OTTHUMG00000032825

Gene info

• Identity
  HGNC:10802
• Gene
  SFTPC
• Long gene name
  surfactant protein C
• Synonyms gene
  • SFTP2
• Synonyms gene name
  • surfactant, pulmonary-associated protein C
• Synonyms
  • SP-C
  • PSP-C
  • SMDP2
  • BRICD6
• Synonyms name
  • BRICHOS domain containing 6
• Locus
  8p21.3
• Discovery year
  1988-05-11
• Entrez gene record
  6440
• Pubmed identfication
  • 1859376
  • 16709565
• RefSeq identity
  • NM_003018
• Classification
  • BRICHOS domain containing
• VEGA ID
  OTTHUMG00000163775

Gene info

• Identity
  HGNC:31838
• Gene
  VCX3B
• Long gene name
  variable charge X-linked 3B
• Synonyms
  • VCX-C
• Locus
  Xp22.31
• Discovery year
  2005-01-11
• Classification
  • Variable charge X/Y family
• VEGA ID
  OTTHUMG00000021106

Gene info

• Identity
  HGNC:6776
• Gene
  MAF
• Long gene name
  MAF bZIP transcription factor
• Synonyms gene name
  • v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog
• Synonyms
  • c-MAF
• Locus
  16q23.2
• Discovery year
  1991-08-01
• Entrez gene record
  4094
• Pubmed identfication
  • 14998484
• Classification
  • Basic leucine zipper proteins
• VEGA ID
  OTTHUMG00000137621

Gene info

• Identity
  HGNC:9982
• Gene
  RFX1
• Long gene name
  regulatory factor X1
• Synonyms gene name
  • regulatory factor X, 1 (influences HLA class II expression)
• Synonyms
  • EF-C
• Synonyms name
  • trans-acting regulatory factor 1
  • enhancer factor C
  • MHC class II regulatory factor RFX
• Locus
  19p13.12
• Discovery year
  1991-11-06
• Entrez gene record
  5989
• Pubmed identfication
  • 1505960
  • 8289803
• RefSeq identity
  • NM_002918
• Classification
  • Regulatory factor X family
• VEGA ID
  OTTHUMG00000181979

Gene info

• Identity
  HGNC:3241
• Gene
  EGR4
• Long gene name
  early growth response 4
• Synonyms
  • NGFI-C
  • PAT133
• Locus
  2p13.2
• Discovery year
  1992-07-27
• Entrez gene record
  1961
• Pubmed identfication
  • 1584812
• RefSeq identity
  • NM_001965
• Classification
  • Zinc fingers C2H2-type
• VEGA ID
  OTTHUMG00000129774

Gene info

• Identity
  HGNC:8547
• Gene
  P4HA2
• Long gene name
  prolyl 4-hydroxylase subunit alpha 2
• Synonyms gene name
  • procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II
  • prolyl 4-hydroxylase, alpha polypeptide II
• Synonyms
  • C-P4Halpha(II)
• Synonyms name
  • 4-PH alpha 2
  • collagen prolyl 4-hydroxylase alpha(II)
• GenBank acession
  • U90441
• Locus
  5q31.1
• Discovery year
  1999-02-23
• Entrez gene record
  8974
• Pubmed identfication
  • 9211872
  • 9149945
• RefSeq identity
  • NM_004199
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000059647

MeSH Data

• Name
  Transduction, Genetic
• Concept
  Scope note: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
• Tree numbers
  • E05.393.350.800
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products