Pure and sensitive AMC tagged fluorogenic substrate for deSUMOylating enzymes.
Purity by SDS-PAGE
Physical form description
hSUMO1-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of hSUMO1. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing hSUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. 380 nm) can be measured. The enhanced purity of hSUMO1-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.
Centrifuge the vial prior to opening.
For Research Use Only! Not to be used in humans
Recombinants or rec. proteins