Recombinant hSUMO1-AMC

• Catalog number
  6412-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Recombinant hSUMO1-AMC
• Description
  Pure and sensitive AMC tagged fluorogenic substrate for deSUMOylating enzymes.
• Recombinant
  Yes
• Purity by SDS PAGE
  ≥95%
• Assay
  RP-HPLC
• Molecular Weight
  11.142 kDa
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Lyophilized powder
• Background Information
  hSUMO1-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of hSUMO1. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing hSUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. 380 nm) can be measured. The enhanced purity of hSUMO1-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  hSUMO1-AMC  
• Short name
  Recombinant hSUMO1-AMC
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Rec. hSUMO1-AMC
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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