Ceramide Kinase (CERK) - Active Enzyme
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Catalog number
X2076E
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Price
Please ask
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Size
10 μg
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Category
Proteins & Peptides
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Long description
Sphingolipids, in addition to being structural components of membranes, regulate cell-cell and cell-substrate interactions, proliferation, and differentiation. Members of this diverse group of lipids have emerged as a novel class of signaling molecules that also regulate phagocytosis. The mechanisms by which sphingolipids exert these effects remain incompletely defined. More than a decade ago, it was found that ceramide can be phosphorylated to ceramide 1-phosphate (C1P). Ceramide kinase (CERK) and its phosphorylated product ceramide 1-phosphate (C1P) are central players in inflammation and cancer. The product of CERK activity, ceramide 1-phosphate (C1P), has been reported to have mitogenic effects. C1P is a direct activator of cytosolic phospholipase A2 and is involved in arachidonic acid release. CERK is a mediator of Ca2+-dependent degranulation in mast cells. In both arachidonic acid release and mast cell degranulation, the intracellular elevation of Ca2+ is a central event that acts as a regulatory mechanism of CERK activity. C1P is found in brain synaptic vesicles, and plays a role in regulating the secretion of neurotransmitters. CERK activity exists in HL-60 cells where the C1P is derived from ceramide released from sphingomyelin. The expressed kinase has specific ceramide phosphorylating activity. CERKs exist in a variety of cellular organisms, including plants, nematodes, insects, and vertebrates.
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Antibody come from
Synthetic protein made to the 3rd cytoplasmic domain of human Muscarinic Acetylcholine Receptor M3 linked to KLH
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Other description
Phosphate buffered saline containing 0.1% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
Ceramide Kinase (CERK) - Active Enzyme
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Antibody is raised in
Sf9 cells
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
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Antibody s suited for
Antibody can be used for immunohistochemistry (5-10µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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Gene target
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Gene symbol
CERK
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Short name
Ceramide Kinase (CERK) - Active Enzyme
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Technique
Enzyme, enzymes
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Isotype
not specified
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Label
unlabeled
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Alternative name
Ceramide phosphorylation catalyst (ceramide kinase) - functionnal Enzyme
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Alternative technique
enzymes
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Alternative to gene target
ceramide kinase, CERK and IDBG-12219 and ENSG00000100422 and 64781, ATP binding, Plasma membranes, Cerk and IDBG-171293 and ENSMUSG00000035891 and 223753, CERK and IDBG-642451 and ENSBTAG00000046654 and
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Gene info
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Identity
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Gene
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Long gene name
ceramide kinase
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Synonyms
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GenBank acession
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Locus
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Discovery year
2002-10-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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