Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in AlzheimerÕs disease including -secretase presenilin and -amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid- . Caspase-12 is ubiquitously expressed in mouse tissues (1).
Antibody come from
Synthetic peptide corresponding to amino acids 2 to 17 of the murine caspase-12
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification
Antigen-antibody binding interaction
Rabbit anti Caspase-12 (NT) (involved in Alzheimers) Antibody
Antibody's reacts with
Human, Mouse, Rat
Antibody's suited for
Detects caspase-12 by Western blot at 0.5 to 1.0 µg/ml. Optimal concentration should be evaluated by serial dilutions.
1. Nakagawa, T., et al., Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 2000, 403, 98-103._x000B__x000B_2. Mehmet, H., Caspases find a new place to hide. Nature 2000, 403, 29-30_x000B__x000B_3. Van de Craen M, et al., Characterization of seven murine caspase family members. FEBS Lett 1997, 403, 61-9
Rabbit anti Caspase-12 (NT) (involved in Alzheimers)
production species: rabbit antibody to caspase-12 (NT) (involved in Alzheimers)