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SPARC Osteonectin human recombinant

SPARC Osteonectin human recombinant is available 2 times from Biovision labs

7204-50 | SPARC/Osteonectin, human recombinant size: 50 ug | 370.84 USD

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7204-10 | SPARC/Osteonectin, human recombinant size: 10 ug | 150.96 USD

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Synonyms Secreted protein acidic and rich in cysteine, BM-40, ON
Alternative_names Secreted protein acidic and rich in cysteine, BM-40, ON
Description Involved in a variety of cellular activities
Recombinant Yes
Source CHO cells
Purity by SDS-PAGE ≥97%
Assay SDS-PAGE
Endotoxin Level< 0.1 ng/ug of protein (
Activity (Specifications/test method) Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5 – 0.7 µg/ml
Biological activity Determined by its ability to increase alkaline phosphatase activity in differentiating MC3T3 cells using a concentration of 0.5 – 0.7 µg/ml
Molecular Weight 43.7 kDa
Storage Temp. -20°C
Shipping Gel Pack
Shelf Life 12 months
Appearance Lyophilized powder
Physical form description Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.6
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Background Information SPARC/Osteonectin is a secreted, evolutionarily conserved collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC/Osteonectin and its related peptides bind to numerous proteins of the extracellular matrix (ECM), affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC/Osteonectin consists of three domains; an N-terminal acidic region that binds calcium ions with low affinity, a module containing two EF-hand motifs that bind calcium with high affinity, and a cysteine-rich follistatin-like domain. Recombinant human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDSPAGE analysis due to the effect of glycosylation
Amino acid sequence APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetSPARC/Osteonectin
Short name SPARC/Osteonectin, recombinant
Technique Recombinant
Species Human
Alternative name secreted protein, acidic, cysteine-rich (osteonectin)/Osteonectin, H. sapiens recombinant
Alternative technique rec
Alternative to gene target secreted protein, acidic, cysteine-rich (osteonectin), ON, SPARC and IDBG-54562 and ENSG00000113140 and 6678, extracellular matrix binding, nuclei, Sparc and IDBG-175174 and ENSMUSG00000018593 and 20692, SPARC and IDBG-646341 and ENSBTAG00000014835 and 282077
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