Ubiquitin, human recombinant
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Catalog number4841-1000
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Price:
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Size1 mg
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SynonymsHuman Ubiquitin
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Alternative_namesUbiquitin
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DescriptionA tag marking proteins for a variety of cellular activities
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Molecular Weight8.6 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLiquid
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Physical form description1 mg/ml in 50 mM HEPES, pH7.5, 150 mM NaCl, 1 mM DTT, and 10% Glycerol.
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Background InformationUbiquitin is a small, evolutionarily conserved eukaryotic protein that can be attached to a wide variety of intraCellular proteins including itself. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target Cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin (Ub) plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The protein to be degraded is conjugated to Ub and the ubiquinated protein is then selectively degraded by a 26S complex, multicatalytic cytosolic and nuclear protease termed proteasome. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous Cellular processes. The recombinant Ubiquitin is expressed from E. coli and purified by proprietary chromatographic techniques.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolUCHL3, UBE2S, UBE2W
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Short nameUbiquitin, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameUbiquitin, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameubiquitin C-terminal hydrolase L3
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Synonyms gene name
- ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin C-terminal hydrolases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin conjugating enzyme E2 S
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Synonyms gene name
- ubiquitin-conjugating enzyme E2S
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-01-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin conjugating enzymes E2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin conjugating enzyme E2 W
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Synonyms gene name
- ubiquitin-conjugating enzyme E2W (putative)
- ubiquitin conjugating enzyme E2 W (putative)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-10-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin conjugating enzymes E2
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data