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V-ATPase, c, vacuolar H+-ATPase, subunit c (16 kDa)

V-ATPase, c, vacuolar H+-ATPase, subunit c (16 kDa) is available 1 time from Agisera labs

AS09 468 | V-ATPase, c, vacuolar H+-ATPase, subunit c (16 kDa) size: 50 µg | 523.58 USD

Catalog number AS09 468
Supplier agisera
Price 523.58 USD
Size 50 µg
Available ordering format Lyophilized in PBS pH 7.4
Immunogen KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit c,Q6IDA4
Raised in Rabbit
Clonality Polyclonal
Clone Polyclonal
Purification Affinity purified serum
How to reconstitute For reconstitution add 50 µl of sterile water
Storage condition store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Verified applications western blot (WB)
Connected products collection of antibodies to vacuolar proteins AS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPaseAS09 503 | anti-V-ATPase, B | vacuolar ATP synthase subunit betaASS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit DAS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPaseAS09 499 | anti-V-ATPase subunit H |V-type proton ATPase subunit HPlant protein extraction bufferSecondary antibodies
Recommended dilutions for use 1: 1000 with standard ECL (WB)
Molecular weight (expected | аpparent) 16 | 16 kDa (Arabidopsis thaliana)
Verified reactivity Arabidopsis thaliana, Mesembryanthemum crystallinum
Possible reactivity dictos including: Cucumis sativus, Gossypium mexicanum, Phaseolus aureus, Raphanus sativus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa
No reactivity algae
Supplementary information Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
References to be added when available, antibody released in June 2014.
Scientific context V-ATPase c subunit is located in vacuole and is involved in ATP synthesis cuopled proton transport. This protein is coded by ATVHA-C3 gene. Alternative names: AT4g34720/T4L20_300
Notes Subunit c is one of most hydrophobic proteins (can be dissolved in organic solvent such as a mixture of chloroform/methanol solution). It is prone to aggregation even in the presence of SDS. Therefore, before loading on the gel membrane fractions should be incubated in buffer containing 2 % SDS at 60° or 70°C for 10 min or at 25°C for 30 min. Protocol of isolation of plant vacuolar membranes can be found here.
Protein number Q6IDA4
TAIR number AT2G16510
Description The kilo Daltons subunit weight of V-ATPase, c, vacuolar H+-ATPase, subunit c (16 ) compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
Gene targetV-ATPase vacuolar ATPase subunit
Short name V-ATPase, c, vacuolar H+-ATPase, subunit c (16 )
Alternative name V-ATPase, c, vacuolar H+-ATPase, functionnal sequence c (16 kiloDalton)
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