V-ATPase, c, vacuolar H+-ATPase, subunit c (16 kDa)

  • Catalog number
    AS09 468
  • Price
    Please ask
  • Size
    50 µg
  • Available ordering format
    Lyophilized in PBS pH 7.4
  • Immunogen
    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit c,Q6IDA4
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified serum
  • How to reconstitute
    For reconstitution add 50 µl of sterile water
  • Storage condition
    store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    collection of antibodies to vacuolar proteins AS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPaseAS09 503 | anti-V-ATPase, B | vacuolar ATP synthase subunit betaASS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit DAS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPaseAS09 499 | anti-V-ATPase subunit H |V-type proton ATPase subunit HPlant protein extraction bufferSecondary antibodies
  • Recommended dilutions for use
    1: 1000 with standard ECL (WB)
  • Molecular weight expected аpparent
    16 | 16 kDa (Arabidopsis thaliana)
  • Verified reactivity
    Arabidopsis thaliana, Mesembryanthemum crystallinum
  • Possible reactivity
    dictos including: Cucumis sativus, Gossypium mexicanum, Phaseolus aureus, Raphanus sativus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa
  • No reactivity
    algae
  • Supplementary information
    Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
  • References
    to be added when available, antibody released in June 2014.
  • Scientific context
    V-ATPase c subunit is located in vacuole and is involved in ATP synthesis cuopled proton transport. This protein is coded by ATVHA-C3 gene. Alternative names: AT4g34720/T4L20_300
  • Notes
    Subunit c is one of most hydrophobic proteins (can be dissolved in organic solvent such as a mixture of chloroform/methanol solution). It is prone to aggregation even in the presence of SDS. Therefore, before loading on the gel membrane fractions should be incubated in buffer containing 2 % SDS at 60° or 70°C for 10 min or at 25°C for 30 min. Protocol of isolation of plant vacuolar membranes can be found here.
  • Protein number
    Q6IDA4
  • TAIR number
    AT2G16510
  • Description
    The kilo Daltons subunit weight of V-ATPase, c, vacuolar H+-ATPase, subunit c (16 ) compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
  • Gene target
  • Short name
    V-ATPase, c, vacuolar H+-ATPase, subunit c (16 )
  • Alternative name
    V-ATPase, c, vacuolar H+-ATPase, functionnal sequence c (16 kiloDalton)
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