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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit c,Q6IDA4
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 50 µl of sterile water
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Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
collection of antibodies to vacuolar proteins AS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPaseAS09 503 | anti-V-ATPase, B | vacuolar ATP synthase subunit betaASS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit DAS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPaseAS09 499 | anti-V-ATPase subunit H |V-type proton ATPase subunit HPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 1000 with standard ECL (WB)
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Molecular weight expected аpparent
16 | 16 kDa (Arabidopsis thaliana)
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Verified reactivity
Arabidopsis thaliana, Mesembryanthemum crystallinum
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Possible reactivity
dictos including: Cucumis sativus, Gossypium mexicanum, Phaseolus aureus, Raphanus sativus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa
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No reactivity
algae
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Supplementary information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
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References
to be added when available, antibody released in June 2014.
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Scientific context
V-ATPase c subunit is located in vacuole and is involved in ATP synthesis cuopled proton transport. This protein is coded by ATVHA-C3 gene. Alternative names: AT4g34720/T4L20_300
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Notes
Subunit c is one of most hydrophobic proteins (can be dissolved in organic solvent such as a mixture of chloroform/methanol solution). It is prone to aggregation even in the presence of SDS. Therefore, before loading on the gel membrane fractions should be incubated in buffer containing 2 % SDS at 60° or 70°C for 10 min or at 25°C for 30 min. Protocol of isolation of plant vacuolar membranes can be found here.
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Protein number
Q6IDA4
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TAIR number
AT2G16510
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Description
The kilo Daltons subunit weight of V-ATPase, c, vacuolar H+-ATPase, subunit c (16 ) compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein