Available ordering format
Lyophilized in PBS pH 7.4
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit c,Q6IDA4
Raised in
Rabbit
Clonality
Polyclonal
Clone
Polyclonal
Purification
Affinity purified serum
How to reconstitute
For reconstitution add 50 µl of sterile water
Storage condition
store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Verified applications
western blot (WB)
Connected products
collection of antibodies to vacuolar proteins AS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPaseAS09 503 | anti-V-ATPase, B | vacuolar ATP synthase subunit betaASS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit DAS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPaseAS09 499 | anti-V-ATPase subunit H |V-type proton ATPase subunit HPlant protein extraction bufferSecondary antibodies
Recommended dilutions for use
1: 1000 with standard ECL (WB)
Molecular weight (expected | аpparent)
16 | 16 kDa (Arabidopsis thaliana)
Verified reactivity
Arabidopsis thaliana, Mesembryanthemum crystallinum
Possible reactivity
dictos including: Cucumis sativus, Gossypium mexicanum, Phaseolus aureus, Raphanus sativus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa
No reactivity
algae
Supplementary information
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
References
to be added when available, antibody released in June 2014.
Scientific context
V-ATPase c subunit is located in vacuole and is involved in ATP synthesis cuopled proton transport. This protein is coded by ATVHA-C3 gene. Alternative names: AT4g34720/T4L20_300
Notes
Subunit c is one of most hydrophobic proteins (can be dissolved in organic solvent such as a mixture of chloroform/methanol solution). It is prone to aggregation even in the presence of SDS. Therefore, before loading on the gel membrane fractions should be incubated in buffer containing 2 % SDS at 60° or 70°C for 10 min or at 25°C for 30 min. Protocol of isolation of plant vacuolar membranes can be found here.
Protein number
Q6IDA4
TAIR number
AT2G16510
Description
The kilo Daltons subunit weight of V-ATPase, c, vacuolar H+-ATPase, subunit c (16 ) compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
Gene targetV-ATPase
vacuolar
ATPase
subunit
Short name
V-ATPase, c, vacuolar H+-ATPase, subunit c (16 )
Alternative name
V-ATPase, c, vacuolar H+-ATPase, functionnal sequence c (16 kiloDalton)
