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CD45 LCA Clone X16 99 Mouse Monoclonal antibody Human

CD45 LCA Clone X16 99 Mouse Monoclonal antibody Human is available 3 times from Accurate-monoclonals labs

MEDCLA414 | CD45, LCA, Clone: X16/99, Mouse Monoclonal antibody-Human, FITC; flow, surface staining size: vial | 1,128.36 USD

Catalog number MEDCLA414
Price 1,128.36 USD
Size vial
Alternative name 1 CD45, LCA, Clone: X16/99, Mab anti-Human, FITC; flow, surface staining
Alternative name 2 CD45, LCA, Clone: X16/99, Mab antibody-Human, FITC; flow, surface staining
Alternative name 3 CD45, LCA, Clone: X16/99, Mab antibody-Homo sapiens, FITC; flow, surface staining
Alternative name 4 anti-CD45 LCA Clone: X16/99 flow surface staining
Alternative name 5 CD45 LCA Clone: X16/99 flow surface staining
Properties If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation Anti-FITC Antibody
Test Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
About Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Latin name Mus musculus
French translation anticorps
Gene targetCD45 LCA X16 Monoclonal surface staining
Short name CD45, LCA, Clone: X16/99, Mouse Monoclonal antibody- FITC; surface staining
Technique Mouse monoclonal, Antibody, Mouse, FITC, Flow
Label FITC
Alternative name CD45, LCA, clonality: X16/99, Mouse monoclonal (Antibody to)-H. sapiens, fluorecein; flow, surface coloring
1. Gene info
Identity 9667
Gene PTPRCAP
Long gene name protein tyrosine phosphatase receptor type C associated protein
Synonyms
  • LPAP
  • CD45-AP
Locus 11q13.2
Discovery year 1996-04-22
Entrez gene record 5790
RefSeq identity
  • NM_005608
Havana BLAST/BLAT OTTHUMG00000150325
2. Gene info
Identity 2090
Gene CLTA
Long gene name clathrin light chain A
Synonyms gene name
  • clathrin, light polypeptide (Lca)
  • clathrin, light chain A
Synonyms
  • Lca
Locus 9p13.3
Discovery year 1991-08-08
Entrez gene record 1211
Pubmed identfication
  • 7713494
RefSeq identity
  • NM_007096
Classification
  • Clathrin subunits
Havana BLAST/BLAT OTTHUMG00000019896
Product images
    #e#.jpg#Mouse#.jpg#Human#.jpg

MEDCLA218-1 | CD45, LCA, Clone: X16/99, Mouse Monoclonal antibody-Human; frozen/paraffin, IH size: 1000ul | 730.76 USD

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MEDCLA218-01 | CD45, LCA, Clone: X16/99, Mouse Monoclonal antibody-Human; frozen/paraffin, IH size: 0.1000ul | 271.15 USD

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Clonality Monoclonal Antibody
Description This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
Storage and handling Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
Advisory To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
Host mouse monoclonal
Species Human
Alternative technique antibodies
Tissue frozen
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CD45, LCA, Clone: X16/99, Mouse Monoclonal antibody- FITC; s
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  • CD45
  • LCA
  • X16
  • Monoclonal
  • surface
  • staining
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