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Rabbit anti Bovine Trypsin

Rabbit anti Bovine Trypsin is available 3 times from Nordc labs

NE157/PAb | Rabbit anti Bovine Trypsin size: 1 mg | 452.20 USD

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NE157/7S | Rabbit anti Bovine Trypsin size: 10 mg | 214.80 USD

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NE157/Bio | Rabbit anti Bovine Trypsin antibody conjugated with Biotin size: 1 mL | 345.93 USD

Catalog number NE157/Bio
Supplier nordc
Price345.93 USD
Size 1 mL
Long description The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
Other description Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Antigen-antibody binding interaction Rabbit anti Bovine Trypsin antibody conjugated with Biotin Antibody
Antibody's specificity Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to trypsin from Bovine pancreas.
Application ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting
Antibody's suited for ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting. Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/5,000.
Storage The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation Biotinylated
About Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name Oryctolagus cuniculus
French translation anticorps
Gene targetTrypsin conjugated with
Short name Rabbit anti Trypsin antibody conjugated with
Technique Antibody, Rabbit, anti
Label Biotin
Alternative name production species: rabbit antibody to Bovine Trypsin (Antibody to) coupled including biotinilated
Alternative technique antibodies
MeSH Data
Concept
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
Additional information
Entrez Genewith trident
Product images
    #Bovine#.png
Category Primary Antibodies
Antibody come from Trypsin isolated and purified from bovine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Antibody is raised in Rabbit
Antibody's reacts with these species Cross-reactivities anti enzymes of other sources may occur but have not been determined.
Antibody's reacts with Bovine
Research interest Enzymes, Veterinary
Relevant references no information yet
Protein number P00760
Warnings This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Clone Polyclonal
Host Rabbit
Species Bovine
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