Centrifuge the vial prior to opening.
For Research Use Only! Not to be used in humans
• Detection method- Absorbance (450 nm) • Species reactivity- human • Application- Quantitative protein detection, establishing normal range etc.
Absorbance (450 nm)
This ELISA kit is used for quantitative protein detection, establishing normal range.
• Serum or plasma
Features & Benefits
• Easy, convenient and time-saving method to assay for human growth hormone.
Human GH (Growth Hormone) has profound effects on tissue growth & metabolism. Hyposecretion of hGH becomes apparent in infants a few months after birth & may result in dwarfism. On the contrary, hypersercretion of hGH results in gigantism. BioVision’s human GH kit is a solid phase sandwich ELISA Kit. The samples, and anti-hGH-HRP conjugate are added to the wells coated with hGH monoclonal antibody. hGH in the sample binds to anti-hGH MAb on the well and the anti-HGH second antibody then binds to hGH. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of hGH in the samples. A standard curve is prepared relating color intensity to the concentration of the hGH. Expected value of hGH in adults (< 10 ng/ml), & in children (< 20 ng/ml).