Kit with Hi-Bind™ Ni QR Agarose Bead columns, & loading, washing & elution buffers for small scale purification of poly-histidine tagged proteins. Protein activity and functional studies rely largely on the availability of purified target proteins. Majority of protein studies are carried out on tagged recombinant proteins expressed in various host organisms. Over 50% of these tagged recombinant proteins are expressed as fusions with poly-histidine purification tags. The small size and the mild conditions utilized during His-tagged protein purification as well as the associated low costs makes this type of fusion the most popular (and in many cases, the first tag of choice). Ready-to-Use Ni-IDA Spin Purification Kit provides an all-inclusive set of reagents required for the small-scale purification of these poly-histidine-tagged proteins rapidly. Each of the Ni-IDA Spin Columns is filled with 100 µL of high performance Hi-Bind™ Ni QR Agarose Beads (Cat. #6562), enabling efficient capture and purification of up to 2.8 mg of poly-histidine tagged proteins per column. The small volume of beads inside the columns is optimal for loading various amounts of sample. At normal expression levels, it permits efficient displacement of non-tagged protein impurities by the more strongly binding poly-histidine tagged targets and improves the purity of the final product. The beads packed in each column deliver as much as 25% higher capacity than Ni-NTA adsorbents while dramatically decreasing purification times due to the ability to perform centrifugation. The kit also includes ready to use column loading, wash and elution buffers aiding in fast purification of the His-tagged proteins. The yield and purity of purification are protein specific and depends on various factors like the expression level, structure, solubility etc.
Salient Features: Binding Capacity: Upto 2.8 mg poly-histidine tagged proteins per column. Column Content: 25 Ni-IDA spin columns, each containing 100 µl high performance Hi-Bind™ Ni QR Agarose Beads (Cat. #6562). Bead content: A 50% slurry (e.g., 1 ml of settled resin is equivalent to 2 ml of 50% slurry) in 20% ethanol. Applications: • Ideal for small scale, one – step purification of poly-histidine tagged recombinant proteins under native conditions. • Ideal for small scale, one – step purification of native proteins and peptides that have an affinity for metal ions. • Screening of expression levels in a HTP manner. • Applicable for purification under native and denaturing conditions.
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
- - Ni-IDA Spin Purification Kit
Ready-to-Use Ni-IDA Spin purification reagent