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Ready to Use Ni Kit

Ready to Use Ni Kit is available 3 times from Biovision labs

K6563-3 | Ready-to-Use Ni QR Agarose Beads Buffer Kit size: 2X3 components | 405.35 USD

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K6563-3 | Ready-to-Use Ni QR Agarose Beads Buffer Kit size: 3 components | 182.41 USD

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K6567-25 | Ready-to-Use Ni-IDA Spin Purification Kit size: 25 Ni-IDA columns, 3 buffers, 25 centrifuge tubes | 387.47 USD

Catalog number K6567-25
Supplier Biovision
Price 387.47 USD
Size 25 Ni-IDA columns, 3 buffers, 25 centrifuge tubes
Taglines Kit with Hi-Bind Ni QR Agarose Bead columns, & loading, washing & elution buffers for small scale purification of poly-histidine tagged proteins
Category Ni-IDA Spin Purification Kit
Test Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.
Gene targetNi-IDA Spin Kit
Short name - - Ni-IDA Spin Purification Kit
Technique Purification
Alternative name Ready-to-Use Ni-IDA Spin purification reagent
Alternative technique purifications
Product images
    Ready-to-Use Ni-IDA Spin Purification Kit
Packaging EA
Storage Temp. +4°C
Shipping gel pack
Shelf Life 12 months
Highlights • Contents: Ni QR Agarose Loading Buffer, Elution Buffer & Extraction Reagent • Sufficient for purification of up to 300 mg of target protein using 5 ml of Hi-Bind™ Ni QR Agarose Beads (Cat. # K6562) or equivalent under batch/gravity flow conditions. • Applications: - Purification of native and recombinant proteins and peptides that have an affinity for metal ions - For purifications under native conditions
Description Basic research of the physico-chemical properties as well as the activity of proteins is impossible without their isolation and purification. Majority of protein studies are carried out on tagged recombinant proteins expressed in various host organisms. Over 50% of these are expressed as fusions with poly-histidine purification tags. The small size and the mild conditions utilized during purification as well as the low cost of purification made this type of fusion the most popular (and in many cases, the first tag of choice). BioVision’s Ready-to-Use Ni QR Agarose Beads Buffer Kit works seamlessly with Hi-Bind™ Ni QR Agarose Beads (Cat. # 6562) or equivalent from the isolation of target to obtaining highly purified and biologically active protein. These Buffers & Extraction Reagent deliver efficient extraction of target protein (as high as 96% efficiency of SDS boiling method) while preserving its integrity. The buffer components are designed to minimize the metal ion leaching while preserving the biological activity of precious target proteins. This kit is sufficient for purification of up to 300 mg of target protein using 5 ml of Hi-Bind™ Ni QR Agarose Beads (Cat. # K6562) or equivalent under batch/gravity flow conditions.
Storage condition +4°C
Shipping condition gel pack
Maximum time for storage 12 months
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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