Cell Meter Flow Cytometric Calcium Assay Kit
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Catalog number
36310
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Price
Please ask
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Size
100 Tests
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Description
Cell Meter Flow Cytometric Calcium Assay Kit is used for detecting intracellular calcium mobilization using a flow cytometer. It can be used for kinetic reading or for endpoint reading.
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Shipping
Room Temperature
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Storage
Freezer
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Shelflife
6 months
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Excitation Wavelength
490 nm
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Emission Wavelength
525 nm
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Personal precautions
Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
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Uses
Laboratory chemicals *For Research Use Only*
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Ordering
To order Cell Meter Flow Cytometric Calcium Assay Kit, please use the Cat. Nr.36310 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
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Tips
Our specialists recommend you to follow carefully the pre-registered instructions for Cell Meter Flow Cytometric Calcium Assay Kit
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Test
aat supplies other types of Assays as 1. Flow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
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Additional description
For cells, cell lines and tissues in culture till half confluency.
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Gene target
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Short name
Meter Cytometric Calcium Assay Kit
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Technique
Assay, Flow, assays
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Host
Assay
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Alternative name
cellular Meter Flow Cytometric Calcium test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissue
cell
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MeSH Data
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Name
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Concept
Scope note:
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
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Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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