Human B-raf Gene Mutations Detection Kit (Multiplex Fluorescence PCR) is used to detect BRAF mutation, in ordre to diagnose colorectal cancer and thyroid cancer. It's recommanded in prior to detect KRAS cancer, if there is no KRAS mutation BRAF detection should be considerid.
Detection Kit for Human BRAF Gene Mutation
Multiplex Fluorescence PCR
Paraffin-embedded tissue, fresh tumor tissue, fresh frozen tumors, blood, pleural effusion, etc
Advantages and Characteristics
Easy operation: This kit only takes one step to add the sample, which can avoid pollution and save time, and it takes only 90 minutes to complete the detection, Strong Specificity: This kit are able to tolerate DNA in the background of 100ng wild-type genome, without nonspecific amplification, High snsitivity: This kit can accurately detect DNA samples with a mutation content of 0.1% in the background of 10ng genome.
The detections of the targets with this kit is a type of test that can be performed on any target containing biological samples after clean up of interfering agents. The assay must be performed following the protocol.cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
Fluorimetric analysis of fluorescent emission of light by is a form of luminescence. The emitted light has a longer wavelength, and therefore lower energy, than the absorbed radiation for excitation. Fluorescent controls and calibrators can be supplied to mix with the cells. A fluorimeter is needed to do the analysis of the green, red, blue, yellow, orange, deep red fluorescent dyes.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
PCR, polymerase chain reaction
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
B-raf Mutations Kit (Multiplex PCR)
detection, multiplex, PCR
H. sapiens B-raf Gene Mutations quantification reagent (Multiplex Fluorescence PCR test reagent)
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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