Neuregulin/Heregulin-1β (NRG-1β/HRG-1β), human recombinant
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Catalog number4711-50
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PricePlease ask
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Size50 ug
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SynonymsNGR beta 1, Heregulin, HRG 1beta, Neuregulin, Neuregulin1 (NRG1-β1), Neu differentiation factor, Pro-neuregulin-1, Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor
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Alternative_namesNGR beta 1, Heregulin, HRG 1beta, Neuregulin, Neuregulin1 (NRG1-β1), Neu differentiation factor, Pro-neuregulin-1, Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor
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DescriptionA polypeptide growth factor
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Purity≥98%
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Biological activityThe ED₅₀ was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 10⁶ units/mg.
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Molecular Weight7.86 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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AppearanceLyophilized protein
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Physical form description< 0.5 ng/ml
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Reconstitution InstructionsReconstitute in H₂O to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
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Background InformationNeuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extraCellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-β1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant human Heregulin-β1 (HRG1-β1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin-β1 (65 amino acid residues).
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolNRG1, SLC48A1, NRG1-IT1, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, HRG-AS1, TRUND-NNN7-1, NRG1-IT3, IGKV1OR2-1
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Short nameNeuregulin/Heregulin-1β (NRG-1β/HRG-1β), recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameNeuregulin/Heregulin-1β (NRG-1β/histidine-rich glycoprotein-1β), H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targethistidine-rich glycoprotein, HPRG and HRGP and THPH11, HRG and IDBG-69039 and ENSG00000113905 and 3273, metal ion binding, Extracellular, Hrg and IDBG-148872 and ENSMUSG00000022877 and 94175, HRG and IDBG-634671 and ENSBTAG00000001265 and 101909599,280820
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Gene info
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Identity
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Gene
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Long gene nameneuregulin 1
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Synonyms gene
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Synonyms gene name
- NRG1 intronic transcript 2 (non-protein coding)
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-03-19
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Entrez gene record
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Pubmed identfication
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Classification
- I-set domain containing
- Neuregulins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesolute carrier family 48 member 1
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Synonyms gene name
- solute carrier family 48 (heme transporter), member 1
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Synonyms
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Locus
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Discovery year2008-10-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Solute carriers
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNRG1 intronic transcript 1
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Synonyms gene name
- NRG1 intronic transcript 1 (non-protein coding)
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GenBank acession
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Locus
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Discovery year2011-12-06
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Classification
- Intronic transcripts
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameHRG and FETUB antisense RNA 1
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Locus
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Discovery year2021-10-11
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Entrez gene record
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Classification
- Antisense RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameNRG1 intronic transcript 3
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Synonyms gene name
- NRG1 intronic transcript 3 (non-protein coding)
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Locus
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Discovery year2011-12-06
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Classification
- Intronic transcripts
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
