Synonyms
NGR beta 1, Heregulin, HRG 1beta, Neuregulin, Neuregulin1 (NRG1-β1), Neu differentiation factor, Pro-neuregulin-1, Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor
Alternative_names
NGR beta 1, Heregulin, HRG 1beta, Neuregulin, Neuregulin1 (NRG1-β1), Neu differentiation factor, Pro-neuregulin-1, Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor
Recombinant
Yes
Source
E. coli
Purity by SDS-PAGE
≥98%
Assay
SDS-PAGE
Purity
≥98%
Biological activity
The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is < 0.5 ng/ml, corresponding to a specific activity of > 2 x 10⁶ units/mg.
Molecular Weight
7.86 kDa
Storage Temp.
-20°C
Shipping
Gel pack
Shelf Life
12 months
Physical form description< 0.5 ng/ml
Reconstitution Instructions
Reconstitute in H₂O to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Background Information
Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extraCellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-β1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant human Heregulin-β1 (HRG1-β1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin-β1 (65 amino acid residues).
Antibody Target
Heregulin 1 beta
Target Alternative Name
NGR beta 1, Heregulin, HRG 1beta, Neuregulin
Antibody Type
Polyclonal
Species Reactivities
Human
Immunogen Sequence
Recombinant protein Heregulin-1 beta (177-241 aa) (BV-M66)
Accession #
Q15491
Gene Id
3084
Form
Liquid
Formulation
0.5 mg/ml in PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin
Purification
Affinity Purified Antibody
Application
Western blot
Positive Control
Western blot: Recombinant protein
Application And Usages
Western blot: ~1:200
Storage Condition
-20 °C
Shipping Condition
gel pack
Western Blot Verified
Verified
Immunocytochemistry Verified
Not Verified
Immunofluorescence Verified
Not Verified
Immunoprecipitation Verified
Not Verified
ELISA Verified
Not Verified
ChIP Verified
Not Verified
Flow Cytometry Verified
Not Verified
