Caspase-9 Substrate LEHD-AFC
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Catalog number1075-200
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PricePlease ask
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Size200 assays
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Alternative_nameAc-LEHD-AFC, Caspase-9 Substrate, Fluorogenic
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DescriptionReady-to-use fluorometric substrate for caspase-9/Mch-6 and related caspases that recognize the amino acid sequence LEHD. Caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEHD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80) and DTT (Cat.# 1201-1) for caspase assays are also available separately.
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Structure AvailableNo
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Peptide sequenceAc-Leu-Glu-His-Asp-AFC
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Salt FormNo
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Molecular FormulaC₃₃H₃₈F₃N₇O₁₁
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Molecular Weight765.7
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Cell PermeableNo
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Purity>98% by HPLC
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HandlingProtect from light and moisture
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Tag LineA caspase-9 substrate
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Storage Condition-20°C
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Shipping Conditiongel pack
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Shelf Life12 months
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MDL NumberMFCD01862610
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PubChem CID44134612
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SMILESCC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)O)C(=O)NC2=CC3=C(C=C2)C(=CC(=O)O3)C(F)(F)F)NC(=O)C
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InChiInChI=1S/C33H38F3N7O11/c1-15(2)8-22(39-16(3)44)31(52)41-21(6-7-26(45)46)29(50)42-23(9-18-13-37-14-38-18)32(53)43-24(12-27(47)48)30(51)40-17-4-5-19-20(33(34,35)36)11-28(49)54-25(19)10-17/h4-5,10-11,13-15,21-24H,6-9,12H2,1-3H3,(H,37,38)(H,39,44)(H,40,51)(H,41,52)(H,42,50)(H,43,53)(H,45,46)(H,47,48)
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InChi KeyHULKIXRFKRCRHD-UHFFFAOYSA-N
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MSDS AvailableYes
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Additional descriptionHuman and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Gene target
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Short nameCaspase-9 Substrate LEHD-AFC
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Alternative namecaspase-9 Substrate LEHD-AFC
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MeSH Data
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Name
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ConceptScope note: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
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